Deep Antimicrobial Activity and Stability Analysis Inform Lysin Sequence-Function Mapping.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00509
Abstract: Antibiotic-resistant infectious disease is a critical challenge to human health. Antimicrobial proteins offer a compelling solution if engineered for potency, selectivity, and physiological stability. Lysins, which lyse cells via degradation of cell wall peptidoglycans, have… read more here.
Keywords: stability; activity; activity stability; sequence function ... See more keywords
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15540-1
Abstract: Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its application to… read more here.
Keywords: sequence function; glms ribozyme; sequence; rna ... See more keywords
Substrate scope expansion of 4-phenol oxidases by rational enzyme selection and sequence-function relations
Sign Up to like & getrecommendations! Published in 2024 at "Communications Chemistry"
DOI: 10.1038/s42004-024-01207-1
Abstract: Enzymes play an important role in organic synthesis thanks to their high selectivity, however, such selectivity limits their substrate scope and hampers their wide application. Here, the authors report an approach to rationally select enzymes… read more here.
Keywords: sequence; phenol; sequence function; substrate scope ... See more keywords
Gauge fixing for sequence-function relationships
Sign Up to like & getrecommendations! Published in 2025 at "PLOS Computational Biology"
DOI: 10.1371/journal.pcbi.1012818
Abstract: Quantitative models of sequence-function relationships are ubiquitous in computational biology, e.g., for modeling the DNA binding of transcription factors or the fitness landscapes of proteins. Interpreting these models, however, is complicated by the fact that… read more here.
Keywords: function relationships; biology; sequence; sequence function ... See more keywords
Elucidation of Sequence–Function Relationships for an Improved Biobutanol In Vivo Biosensor in E. coli
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.821152
Abstract: Transcription factor (TF)–promoter pairs have been repurposed from native hosts to provide tools to measure intracellular biochemical production titer and dynamically control gene expression. Most often, native TF–promoter systems require rigorous screening to obtain desirable… read more here.
Keywords: biosensor; sort seq; function; promoter ... See more keywords