Deep Antimicrobial Activity and Stability Analysis Inform Lysin Sequence-Function Mapping.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00509
Abstract: Antibiotic-resistant infectious disease is a critical challenge to human health. Antimicrobial proteins offer a compelling solution if engineered for potency, selectivity, and physiological stability. Lysins, which lyse cells via degradation of cell wall peptidoglycans, have… read more here.
Keywords: stability; activity; activity stability; sequence function ... See more keywords
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15540-1
Abstract: Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its application to… read more here.
Keywords: sequence function; glms ribozyme; sequence; rna ... See more keywords
Elucidation of Sequence–Function Relationships for an Improved Biobutanol In Vivo Biosensor in E. coli
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.821152
Abstract: Transcription factor (TF)–promoter pairs have been repurposed from native hosts to provide tools to measure intracellular biochemical production titer and dynamically control gene expression. Most often, native TF–promoter systems require rigorous screening to obtain desirable… read more here.
Keywords: biosensor; sort seq; function; promoter ... See more keywords