Ancestral Sequence Reconstruction and Comprehensive Computational Simulations Unmask an Efficient PET Hydrolase with the Wobbled Catalytic Triad.
Sign Up to like & getrecommendations! Published in 2025 at "ChemSusChem"
DOI: 10.1002/cssc.202402614
Abstract: Beyond directed evolution, ancestral sequence reconstruction (ASR) has emerged as a powerful strategy for engineering proteins with superior functional properties. Herein, we harnessed ASR to uncover robust PET hydrolase variants, expanding the repertoire of PET-degrading… read more here.
Keywords: sequence; ancestral sequence; pet hydrolase; sequence reconstruction ... See more keywords
Ancestral Sequence Reconstruction of the Ethylene-Forming Enzyme
Sign Up to like & getrecommendations! Published in 2025 at "Biochemistry"
DOI: 10.1021/acs.biochem.5c00334
Abstract: The ethylene-forming enzyme (EFE) catalyzes two main reactions: the conversion of 2-oxoglutarate (2OG) to ethylene plus CO2 and the oxidative decarboxylation of 2OG coupled to the C5 hydroxylation of l-arginine (l-Arg). EFE also facilitates two… read more here.
Keywords: reconstruction ethylene; forming enzyme; ancestral sequence; arg ... See more keywords
Engineering the Thermostability of a d-Carbamoylase Based on Ancestral Sequence Reconstruction for the Efficient Synthesis of d-Tryptophan.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c07781
Abstract: Employing ancestral sequence reconstruction and consensus sequence analysis, the thermostability of a novel d-carbamoylase derived from Nitratireductor indicus (NiHyuC) was engineered through greedy-oriented iterative combinatorial mutagenesis. A mutant S202P/E208D/R277L (M4Th3) was obtained with significantly elevated… read more here.
Keywords: ancestral sequence; carbamoylase; sequence; thermostability ... See more keywords
Unveiling Highly Active and Stable l-Glutaminase through Ancestral Sequence Reconstruction and Turnover Number Prediction.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c11502
Abstract: In our study, we employed ancestral sequence reconstruction and DLKcat analysis to engineer l-glutaminases with enhanced activity and thermal stability, using Bacillus subtilis 168 l-glutaminase (YbgJ) as the template. We identified two ancestral l-glutaminases, Anc165… read more here.
Keywords: anc165; sequence reconstruction; ancestral sequence; activity ... See more keywords
Stable and Promiscuous Galactose Oxidases Engineered by Directed Evolution, Atomistic Design, and Ancestral Sequence Reconstruction
Sign Up to like & getrecommendations! Published in 2024 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.4c00653
Abstract: Galactose oxidase (GOase) is a versatile biocatalyst with a wide range of potential applications, ranging from synthetic chemistry to bioelectrochemical devices. Previous GOase engineering by directed evolution generated the M-RQW mutant, with unprecedented new-to-nature oxidation… read more here.
Keywords: chemistry; ancestral sequence; directed evolution; sequence reconstruction ... See more keywords
Evolutionary insights into the stereoselectivity of imine reductases based on ancestral sequence reconstruction
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-54613-3
Abstract: The stereoselectivity of enzymes plays a central role in asymmetric biocatalytic reactions, but there remains a dearth of evolution-driven biochemistry studies investigating the evolutionary trajectory of this vital property. Imine reductases (IREDs) are one such… read more here.
Keywords: evolution; ancestral sequence; imine reductases; biochemistry ... See more keywords
Deciphering the evolution of flavin-dependent monooxygenase stereoselectivity using ancestral sequence reconstruction.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2218248120
Abstract: Controlling the selectivity of a reaction is critical for target-oriented synthesis. Accessing complementary selectivity profiles enables divergent synthetic strategies, but is challenging to achieve in biocatalytic reactions given enzymes' innate preferences of a single selectivity.… read more here.
Keywords: flavin dependent; ancestral sequence; reaction; stereoselectivity ... See more keywords
QuASeR: Quantum Accelerated de novo DNA sequence reconstruction
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0249850
Abstract: In this article, we present QuASeR, a reference-free DNA sequence reconstruction implementation via de novo assembly on both gate-based and quantum annealing platforms. This is the first time this important application in bioinformatics is modeled… read more here.
Keywords: sequence reconstruction; dna sequence; quantum; quaser ... See more keywords
Preparation and Characterization of an Ancient Aminopeptidase Obtained from Ancestral Sequence Reconstruction for L-Carnosine Synthesis
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27196620
Abstract: As a biologically active peptide, L-carnosine has been widely used in the pharmaceutical, cosmetic and health care industries due to its various physiological properties. However, relatively little research is available regarding L-carnosine’s enzymatic synthesis function.… read more here.
Keywords: carnosine; ancestral sequence; carnosine synthesis; synthesis ... See more keywords