Sequence‐specific targeting of chromatin remodelers organizes precisely positioned nucleosomes throughout the genome
Sign Up to like & getrecommendations! Published in 2017 at "BioEssays"
DOI: 10.1002/bies.201600183
Abstract: Eukaryotic genomes are functionally organized into chromatin, a compact packaging of nucleoproteins with the basic repeating unit known as the nucleosome. A major focus for the chromatin field has been understanding what rules govern nucleosome… read more here.
Keywords: throughout genome; sequence specific; chromatin; positioned nucleosomes ... See more keywords
Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6716-2_16
Abstract: In mammals, transcription in the zygote begins after fertilization. This transcriptional wave is called zygotic gene activation (ZGA). During ZGA, epigenetic modifications, such as DNA methylation and histone modifications, are dynamically and drastically reconstructed in… read more here.
Keywords: sequence specific; noncoding rnas; promoter; associated noncoding ... See more keywords
1H, 15N and 13C sequence specific backbone assignment of the vanadate inhibited hematopoietic tyrosine phosphatase
Sign Up to like & getrecommendations! Published in 2018 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-017-9770-7
Abstract: The sequence-specific backbone assignment of hematopoietic protein tyrosine phosphatase (HePTP; PTPN7) in presence of vanadate has been determined, based on triple-resonance experiments using uniformly [13C,15N]-labeled protein. These assignments facilitate further studies of HePTP in the… read more here.
Keywords: sequence specific; tyrosine phosphatase; tyrosine; backbone assignment ... See more keywords
Sequence specific assignment and determination of OSR1 C-terminal domain structure by NMR.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.03.065
Abstract: The binding of SPAK and OSR1 kinases to their upstream WNK kinases is mediated by the interaction of their highly conserved SPAK and OSR1 C-terminal domain (CTD) to RFx [V/I] peptide sequences from WNK kinases.… read more here.
Keywords: osr1 ctd; osr1 terminal; terminal domain; sequence specific ... See more keywords
DNA sequence-specific dimeric bisbenzimidazoles DBP(n) and DBPA(n) as inhibitors of H-NS silencing in bacterial cells.
Sign Up to like & getrecommendations! Published in 2018 at "Microbiological research"
DOI: 10.1016/j.micres.2017.11.007
Abstract: DNA sequence-specific fluorescent dimeric bisbenzimidazoles DBP(n) and DBPA(n), noncovalently interacting with A-T pairs in the minor groove of double-stranded DNA were used for studying and monitoring the expression of histone-like H-NS-dependent promoters. Histone-like H-NS selectively… read more here.
Keywords: sequence specific; bisbenzimidazoles dbp; dbp dbpa; dimeric bisbenzimidazoles ... See more keywords
Digital Droplet Loop-Mediated Isothermal Amplification Featuring a Molecular Beacon Assay, 3D Printed Droplet Generation, and Smartphone Imaging for Sequence-Specific DNA Detection.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02979
Abstract: Nucleic acid detection is widely used in the amplification and quantitation of nucleic acids from biological samples. While polymerase chain reaction (PCR) enjoys great popularity, expensive thermal cyclers are required for precise temperature control. Loop-mediated… read more here.
Keywords: printed droplet; dna; sequence specific; sequence ... See more keywords
Peptide Retention Time Prediction in Hydrophilic Interaction Liquid Chromatography: Data Collection Methods and Features of Additive and Sequence-Specific Models.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00537
Abstract: The development of a peptide retention prediction model for hydrophilic interaction liquid chromatography (XBridge Amide column) is described for a collection of ∼40 000 tryptic peptides. Off-line 2D LC-MS/MS analysis (HILIC-RPLC) of S. cerevisiae whole cell… read more here.
Keywords: sequence specific; hydrophilic interaction; peptide retention; retention ... See more keywords
A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/d0cc00397b
Abstract: Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM sequence can be recognized… read more here.
Keywords: loop mediated; sequence specific; plasmonic loop; cas12a ... See more keywords
Controlling liquid-liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc04366a
Abstract: We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to… read more here.
Keywords: specific manner; liquid phase; quadruplex forming; phase separation ... See more keywords
Sequence-specific RNA recognition by an RGG motif connects U1 and U2 snRNP for spliceosome assembly
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2114092119
Abstract: Significance Pre-messenger RNA (pre-mRNA) splicing is a key regulatory step in gene expression. The splicing reaction is mediated by the spliceosome, a dynamic complex comprising five small nuclear ribonucleoproteins (snRNPs), which assembles onto each intron… read more here.
Keywords: spliceosome assembly; rgg; sequence specific; sequence ... See more keywords
Sequence-specific minimizers via polar sets.
Sign Up to like & getrecommendations! Published in 2021 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btab313
Abstract: MOTIVATION Minimizers are efficient methods to sample k-mers from genomic sequences that unconditionally preserve sufficiently long matches between sequences. Well-established methods to construct efficient minimizers focus on sampling fewer k-mers on a random sequence and… read more here.
Keywords: sketch size; polar sets; sequence specific; reference ... See more keywords