Nanoliter-scale next-generation sequencing library-mediated high-throughput 16S rRNA microbial community profiling.
Sign Up to like & getrecommendations! Published in 2020 at "BioTechniques"
DOI: 10.2144/btn-2019-0102
Abstract: An ultra-high-throughput workflow for next-generation sequencing library construction at nanoliter scale for amplicon sequencing, termed Smartchip Nanowell Platform for Target Enrichment, was established using a nanodispenser system and a nanoliter-scale PCR chip. To demonstrate its… read more here.
Keywords: sequencing library; microbial community; community profiling; nanoliter scale ... See more keywords
Evaluation of Sequencing Library Preparation Protocols for Viral Metagenomic Analysis from Pristine Aquifer Groundwaters
Sign Up to like & getrecommendations! Published in 2019 at "Viruses"
DOI: 10.3390/v11060484
Abstract: Viral ecology of terrestrial habitats is yet-to be extensively explored, in particular the terrestrial subsurface. One problem in obtaining viral sequences from groundwater aquifer samples is the relatively low amount of virus particles. As a… read more here.
Keywords: sequencing library; library preparation; aquifer; pristine ... See more keywords