Assessment of two-pool multiplex long-amplicon nanopore sequencing of SARS-CoV-2.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of medical virology"
DOI: 10.1002/jmv.27336
Abstract: Genomic surveillance of SARS-CoV-2 plays an important role in COVID-19 pandemic control and elimination efforts, especially by elucidating its global transmission network and illustrating its viral evolution. The deployment of multiplex PCR assays that target… read more here.
Keywords: depth; long amplicon; nanopore sequencing; sars cov ... See more keywords
Genome sequencing of SARS-CoV-2 reveals the prevalence of variant B 1.1.7 in Egypt
Sign Up to like & getrecommendations! Published in 2021 at "Infection, Genetics and Evolution"
DOI: 10.1016/j.meegid.2021.105191
Abstract: Recently, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) resulted in Coronavirus Disease 2019 (COVID-19) outbreak. A new SARS-CoV-2 strain is expected to emerge in late 2020, including B.1.1.7. The high transmission rate of SARS-CoV-2… read more here.
Keywords: sequencing sars; variant egypt; sars cov; reveals prevalence ... See more keywords
Revealing COVID-19 transmission in Australia by SARS-CoV-2 genome sequencing and agent-based modeling
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DOI: 10.1038/s41591-020-1000-7
Abstract: In January 2020, a novel betacoronavirus (family Coronaviridae), named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was identified as the etiological agent of a cluster of pneumonia cases occurring in Wuhan City, Hubei Province, China1,2.… read more here.
Keywords: genome sequencing; transmission; agent based; sars cov ... See more keywords
A rapid, cost-effective tailed amplicon method for sequencing SARS-CoV-2
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DOI: 10.1186/s12864-020-07283-6
Abstract: Background The global COVID-19 pandemic has led to an urgent need for scalable methods for clinical diagnostics and viral tracking. Next generation sequencing technologies have enabled large-scale genomic surveillance of SARS-CoV-2 as thousands of isolates… read more here.
Keywords: amplicon method; tailed amplicon; sequencing sars; method sequencing ... See more keywords
Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples
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DOI: 10.1186/s13073-020-00751-4
Abstract: Background COVID-19 (coronavirus disease 2019) has caused a major epidemic worldwide; however, much is yet to be known about the epidemiology and evolution of the virus partly due to the scarcity of full-length SARS-CoV-2 (severe… read more here.
Keywords: directly clinical; sequencing sars; amplicon; sars cov ... See more keywords