Sequencing of Small DNA Fragments with Aggregated-Induced-Emission Molecule Labelled Nucleotides.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c00707
Abstract: Sequencing by synthesis is a significant method for high-throughput DNA sequencing. Herein, we synthesized terminal aggregated-induced-emission luminogen (AIEgen) labelled nucleotides (dNTPs-HCAP) that could serve as substrates for some polymerases and applied them into the sequencing… read more here.
Keywords: labelled nucleotides; dna; sequencing small; small dna ... See more keywords
Small-seq for single-cell small-RNA sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Nature Protocols"
DOI: 10.1038/s41596-018-0049-y
Abstract: Small RNAs participate in several cellular processes, including splicing, RNA modification, mRNA degradation, and translational arrest. Traditional methods for sequencing small RNAs require a large amount of cell material, limiting the possibilities for single-cell analyses.… read more here.
Keywords: single cell; seq single; sequencing small; small seq ... See more keywords
DNA sequencing of the small bowel adenocarcinomas to identify targetable ErbB2 mutations.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2017.35.15_suppl.e15800
Abstract: e15800Background: Small bowel adenocarcinoma (SBA) is a rare malignancy of the digestive tract with limited knowledge about its genetic alterations. Methods: Eighteen tumor and normal matched samples were sequenced using the whole-exome-Illumina platform. Various agnostic… read more here.
Keywords: small bowel; dna sequencing; sequencing small; erbb2 mutations ... See more keywords
High-Throughput Sequencing of Small RNAs for the Sanitary Certification of Viruses in Grapevine
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2021.682879
Abstract: Biological indexing is the method generally recognized for the certification of propagative grapevines in many countries, and it is mandatory in the European Union. It consists of the evaluation of the plant material after grafting… read more here.
Keywords: sequencing small; small rnas; throughput sequencing; certification ... See more keywords
Deep Sequencing of Small RNA Reveals the Molecular Regulatory Network of AtENO2 Regulating Seed Germination
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22105088
Abstract: Seed germination is a key step in the new life cycle of plants. In agriculture, we regard the rapid and consistent process of seed germination as one of the necessary conditions to measure the high… read more here.
Keywords: seed; seed germination; sequencing small; ath ... See more keywords