Articles with "serial block" as a keyword



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The complex synaptic pathways onto a looming‐detector neuron revealed using serial block‐face scanning electron microscopy

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Published in 2021 at "Journal of Comparative Neurology"

DOI: 10.1002/cne.25227

Abstract: The ability of locusts to detect looming stimuli and avoid collisions or predators depends on a neuronal circuit in the locust's optic lobe. Although comprehensively studied for over three decades, there are still major questions… read more here.

Keywords: face scanning; neuron; microscopy; block face ... See more keywords
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Three‐dimensional fine structure of fibroblasts and other mesodermally derived tissues in the dermis of adults of the Bahamas lancelet (Chordata, Cephalohordata), as seen by serial block‐face scanning electron microscopy

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Published in 2022 at "Journal of Morphology"

DOI: 10.1002/jmor.21502

Abstract: Tissues of adult cephalochordates include sparsely distributed fibroblasts. Previous work on these cells has left unsettled such questions as their developmental origin, range of functions, and even their overall shape. Here, we describe fibroblasts of… read more here.

Keywords: block face; microscopy; bahamas lancelet; face scanning ... See more keywords
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Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy.

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Published in 2020 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0767-1_7

Abstract: This chapter describes methods to enhanced contrast of plant material processed by high-pressure freezing and freeze substitution for improved visualization by serial block-face scanning electron microscopy (SBEM). The contrast enhancing steps are based on a… read more here.

Keywords: plant; microscopy; serial block; pressure freezing ... See more keywords
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Podocyte penetration of the glomerular basement membrane to contact on the mesangial cell at the lesion of mesangial interposition in lupus nephritis: a three-dimensional analysis by serial block-face scanning electron microscopy

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Published in 2019 at "Clinical and Experimental Nephrology"

DOI: 10.1007/s10157-019-01701-0

Abstract: BackgroundThe interaction among the glomerular components plays an important role in the development of glomerular lesions; thus, investigation of the ultrastructural three-dimensional (3D) configuration of the human glomerular cells and extracellular matrix (ECM) is important… read more here.

Keywords: three dimensional; microscopy; face scanning; serial block ... See more keywords
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Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture.

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Published in 2018 at "Methods in cell biology"

DOI: 10.1016/bs.mcb.2018.03.010

Abstract: The mitotic spindle is a complex structure that coordinates the accurate segregation of chromosomes during cell division. To understand how the mitotic spindle operates at the molecular level, high resolution imaging is needed. Serial block… read more here.

Keywords: resolution; microscopy; sbf sem; electron microscopy ... See more keywords
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Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events.

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Published in 2019 at "Methods in cell biology"

DOI: 10.1016/bs.mcb.2019.03.014

Abstract: Volume electron microscopy allows for the automated acquisition of serial-section imaging data that can be reconstructed in three-dimensions (3D) to provide a detailed, geometrically accurate view of cellular ultrastructure. Two, volume electron microscopy (EM) techniques,… read more here.

Keywords: electron microscopy; microscopy; scanning electron; serial block ... See more keywords
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3D microstructure reconstruction of casting aluminum alloy based on serial block-face scanning electron microscopy

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Published in 2019 at "Journal of Alloys and Compounds"

DOI: 10.1016/j.jallcom.2018.11.188

Abstract: Abstract In this work, the inner microstructure of aluminum-silicon casting alloy was experimentally investigated to reveal the spatial morphology and phase distribution. Its industrial use is as a light-weight structural material. As a powerful three-dimensional… read more here.

Keywords: electron microscopy; microscopy; face scanning; scanning electron ... See more keywords
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Determination of secretory granule maturation times in pancreatic islet β-cells by serial block face scanning electron microscopy.

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Published in 2020 at "Journal of structural biology"

DOI: 10.1016/j.jsb.2020.107584

Abstract: It is shown how serial block-face electron microscopy (SBEM) of insulin-secreting β-cells in wild-type mouse pancreatic islets of Langerhans can be used to determine maturation times of secretory granules. Although SBEM captures the β -cell… read more here.

Keywords: secretory; microscopy; electron microscopy; serial block ... See more keywords
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A Semi-Automated Workflow for Segmenting Contents of Single Cardiac Cells from Serial-Block-Face Scanning Electron Microscopy Data

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Published in 2017 at "Microscopy and Microanalysis"

DOI: 10.1017/s143192761700188x

Abstract: Serial-block-face scanning electron microscopy (SBFSEM) has revolutionized electron microscopy of cellular ultrastructure by providing a high throughput method to image large fields of view at tens of nanometer (nm) resolution. These data are enabling scientists… read more here.

Keywords: microscopy; electron microscopy; face scanning; serial block ... See more keywords
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Serial block‐face scanning electron microscopy of erythrocytes protruding through the human placental syncytiotrophoblast

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Published in 2017 at "Journal of Anatomy"

DOI: 10.1111/joa.12658

Abstract: The syncytiotrophoblast forms a continuous barrier between the maternal and fetal circulations. Here we present a serial block‐face scanning electron microscopy (SBFSEM) study, based on a single image stack, showing pooling of fetal blood underneath… read more here.

Keywords: microscopy; syncytiotrophoblast; face scanning; serial block ... See more keywords