Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-92687-x
Abstract: The serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dynamics by time-resolved studies. The fixed-target (FT)… read more here.
Keywords: fixed target; sample holder; polyimide mesh; serial crystallography ... See more keywords
2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography
Sign Up to like & getrecommendations! Published in 2021 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2100170118
Abstract: Significance The 2′-O methyl group in Cap-1 is essential to protect viral RNA from host interferon-induced response. We determined crystal structures of SARS-CoV-2 Nsp10/16 heterodimer in complex with substrates (Cap-0 analog and S-adenosyl methionine) and… read more here.
Keywords: methylation; rna; serial crystallography; sars cov ... See more keywords
ClickX: a visualization-based program for preprocessing of serial crystallography data
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Applied Crystallography"
DOI: 10.1107/s1600576719005363
Abstract: A Python-based program for serial crystallography experimental data preprocessing is developed for both online and offline analysis. Enhanced features include a graphical user interface, batch job execution and fast parameter optimizations. read more here.
Keywords: visualization based; crystallography; based program; serial crystallography ... See more keywords
Serial crystallography using automated drop dispensing
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Synchrotron Radiation"
DOI: 10.1107/s1600577521006160
Abstract: The development of automated sample delivery and drop on demand methods is described. read more here.
Keywords: using automated; drop; automated drop; drop dispensing ... See more keywords
Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography
Sign Up to like & getrecommendations! Published in 2020 at "IUCrJ"
DOI: 10.1107/s2052252520013238
Abstract: A microcrystal-prelocation method is demonstrated using low-dose 2D full-field propagation-based X-ray phase-contrast imaging on samples with an essentially flat geometry for automated serial crystallography data collection at a microfocus macromolecular crystallography beamline. read more here.
Keywords: crystallography; low dose; phase contrast; serial crystallography ... See more keywords
Hit and Indexing Rate in Serial Crystallography: Incomparable Statistics
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.858815
Abstract: Serial crystallography (SX) using X-ray free-electron lasers (XFEL) and synchrotron X-rays is an emerging X-ray crystallography technique to determine the structure of macromolecules at room temperature or near-physiological temperature with minimal radiation damage (Chapman et… read more here.
Keywords: chapman; crystallography; diffraction; hit images ... See more keywords
Sample Delivery Media for Serial Crystallography
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20051094
Abstract: X-ray crystallographic methods can be used to visualize macromolecules at high resolution. This provides an understanding of molecular mechanisms and an insight into drug development and rational engineering of enzymes used in the industry. Although… read more here.
Keywords: sample delivery; delivery; serial crystallography; crystallography ... See more keywords