High fidelity one‐pot DNA assembly using orthogonal serine integrases
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200411
Abstract: Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is… read more here.
Keywords: serine integrases; dna assembly; high fidelity;
Making serine integrases work for us.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in microbiology"
DOI: 10.1016/j.mib.2017.04.006
Abstract: DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements. Serine integrases are a group of recombinases… read more here.
Keywords: dna; serine integrases; making serine; microbiology ... See more keywords
Application of serine integrases for secondary metabolite pathway assembly in Streptomyces
Sign Up to like & getrecommendations! Published in 2020 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2020.05.006
Abstract: Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can… read more here.
Keywords: serine; pathway assembly; application serine; serine integrases ... See more keywords