A Novel Synthetic, Xeno‐Free Biomimetic Surface for Serum‐Free Expansion of Human Mesenchymal Stromal Cells
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Biosystems"
DOI: 10.1002/adbi.202000008
Abstract: Human mesenchymal stromal cells (hMSCs) have enormous potential for the treatment of various inflammatory and degenerative diseases. Their manufacturing for cell‐based therapies requires extensive ex vivo expansion and optimal growth conditions. To support cell adhesion,… read more here.
Keywords: human mesenchymal; mesenchymal stromal; expansion; stromal cells ... See more keywords
A study of the THP‐1 cell line as the potential biologics production platform with the emphasis on serum‐free media substitution for economic expediency
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200154
Abstract: Cell cultures are frequently preferred in the industrial production of high value‐added biopharmaceuticals on a large scale. In the production of biopharmaceuticals, the selection of the appropriate cell line, the cell culture medium, and the… read more here.
Keywords: cell; cell line; thp; serum free ... See more keywords
Nuclear and cytoplasmic quality of oocytes derived from serum‐free culture of secondary follicles in vitro
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.30232
Abstract: In vitro culture of follicles is a promising technology to generate large quantities of mature oocytes and it could offer a novel option of assisted reproductive technologies. Here we described a 2‐dimensional follicular serum‐free culture… read more here.
Keywords: matured follicular; secondary follicles; oocytes matured; free culture ... See more keywords
Defined serum-free culture of human infant small intestinal organoids with predetermined doses of Wnt3a and R-spondin1 from surgical specimens
Sign Up to like & getrecommendations! Published in 2021 at "Pediatric Surgery International"
DOI: 10.1007/s00383-021-04957-4
Abstract: Refinement of organoid technology is important for studying physiology and disease of the intestine. We aimed to optimize defined serum-free conditions for human infant small intestinal (SI) organoid culture with predetermined doses of Wnt3a and… read more here.
Keywords: infant small; defined serum; human infant; doses wnt3a ... See more keywords
Involvement of SNARE Protein Interaction for Non-classical Release of DAMPs/Alarmins Proteins, Prothymosin Alpha and S100A13
Sign Up to like & getrecommendations! Published in 2020 at "Cellular and Molecular Neurobiology"
DOI: 10.1007/s10571-020-00950-y
Abstract: Prothymosin alpha (ProTα) is involved in multiple cellular processes. Upon serum-free stress, ProTα lacking a signal peptide sequence is non-classically released from C6 glioma cells as a complex with Ca 2+ -binding cargo protein S100A13.… read more here.
Keywords: snare; syt; serum free; s100a13 ... See more keywords
A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes
Sign Up to like & getrecommendations! Published in 2018 at "Cytotechnology"
DOI: 10.1007/s10616-018-0289-2
Abstract: AbstracthiHep is a new type of hepatocyte-like cell that is predicted to be a potential unlimited source of hepatocytes for a bioartificial liver. However, hiHep cannot currently be used in clinical settings because serum must… read more here.
Keywords: liver specific; medium; free medium; serum free ... See more keywords
Comparative Analysis of MSC-Derived Exosomes Depending on Cell Culture Media for Regenerative Bioactivity.
Sign Up to like & getrecommendations! Published in 2021 at "Tissue engineering and regenerative medicine"
DOI: 10.1007/s13770-021-00352-1
Abstract: BACKGROUND In order to produce and isolate the exosome derived from the cell of interests, a serum free environment (starvation) has been essential for excluding the unknown effect from serum-derived exosomes. Recently, serum-free culture media… read more here.
Keywords: cell culture; serum free; culture media; cell ... See more keywords
An optimal serum-free defined condition for in vitro culture of kidney organoids.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.05.098
Abstract: Kidney organoid is an emerging topic of importance for research in kidney development and regeneration. Conventional culture systems for kidney organoids reported thus far use culture media containing serum, which may compromise our understanding and… read more here.
Keywords: ksr; kidney organoids; condition; serum free ... See more keywords
Reference intervals and diagnostic ranges for serum free κ and free λ immunoglobulin light chains vary by instrument platform: Implications for classification of patient results in a multi-center study.
Sign Up to like & getrecommendations! Published in 2018 at "Clinical biochemistry"
DOI: 10.1016/j.clinbiochem.2018.06.003
Abstract: BACKGROUND Serum free light chain (FLC) immunoglobulins are key biomarkers that aid in the diagnosis, prognosis and assessment of treatment response in patients with plasma cell disorders (PCD). Here we investigated the transference of manufacturer's… read more here.
Keywords: reference intervals; manufacturer; diagnostic ranges; reference ... See more keywords
Serum Free Methylated Glutathione S-transferase 1 DNA Levels, Survival, and Response to Docetaxel in Metastatic, Castration-resistant Prostate Cancer: Post Hoc Analyses of Data from a Phase 3 Trial.
Sign Up to like & getrecommendations! Published in 2018 at "European urology"
DOI: 10.1016/j.eururo.2018.11.001
Abstract: BACKGROUND Glutathione S-transferase 1 (GSTP1) expression is inactivated in >90% of all prostate cancers in association with aberrant DNA methylation. Detection of serum free methylated GSTP1 (mGSTP1) DNA is associated with overall survival (OS) and… read more here.
Keywords: mgstp1; dna; prostate cancer; serum free ... See more keywords
Platform for the growth and propogation of HEK293 cells and adenovirus viral vector amplification
Sign Up to like & getrecommendations! Published in 2019 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2019.03.332
Abstract: Background & Aim Industrialization of viral vector production for gene therapy applications demands consistent processes utilizing robust production platforms and raw materials that can meet quality standards and regulatory compliance. Typical vector production schemes involve… read more here.
Keywords: bioreactor; growth; viral vector; amplification ... See more keywords