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   Articles with "sgrna" as a keyword



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Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae.

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recommendations! Published in 2018 at "Biotechnology journal"

DOI: 10.1002/biot.201700582

Abstract: Metabolic engineering typically utilizes a suboptimal step-wise gene target optimization approach to parse a highly connected and regulated cellular metabolism. While the endonuclease-null CRISPR/Cas system has enabled gene expression perturbations without genetic modification, it has… read more here.

Keywords: sgrna; operons lego; ligation; approach ... See more keywords
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Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection

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recommendations! Published in 2021 at "Cell Reports"

DOI: 10.1016/j.celrep.2021.109108

Abstract: SARS-CoV-2 uses subgenomic (sg)RNA to produce viral proteins for replication and immune evasion. We applied long-read RNA and cDNA sequencing to in vitro human and primate infection models to study transcriptional dynamics. Transcription-regulating sequence (TRS)-dependent… read more here.

Keywords: sgrna; transcriptional dynamics; transcriptional epi; epi transcriptional ... See more keywords
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ExsgRNA: reduce off-target efficiency by on-target mismatched sgRNA

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recommendations! Published in 2022 at "Briefings in bioinformatics"

DOI: 10.1093/bib/bbac183

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology has been widely used to facilitate efficient genome editing. Current popular sgRNA design tools only consider the sgRNA perfectly matched to the target site and… read more here.

Keywords: exsgrna reduce; target; reduce target; efficiency target ... See more keywords

NbPsbO1 Interacts Specifically with the Bamboo Mosaic Virus (BaMV) Subgenomic RNA (sgRNA) Promoter and Is Required for Efficient BaMV sgRNA Transcription

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recommendations! Published in 2021 at "Journal of Virology"

DOI: 10.1128/jvi.00831-21

Abstract: Production of subgenomic RNAs (sgRNAs) for efficient translation of downstream viral proteins is one of the major strategies adapted for viruses that contain a multicistronic RNA genome. Both viral genomic RNA (gRNA) replication and sgRNA… read more here.

Keywords: sgrna promoter; sgrna transcription; bamv; sgrna ... See more keywords

Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars

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recommendations! Published in 2018 at "Frontiers in Plant Science"

DOI: 10.3389/fpls.2018.00594

Abstract: In an effort to produce reliably contained transgenic trees, we used the CRISPR/Cas9 system to alter three genes expected to be required for normal flowering in poplar (genus Populus). We designed synthetic guide RNAs (sgRNAs)… read more here.

Keywords: sgrna; crispr cas9; spectra among; mutation spectra ... See more keywords
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TRUE Gene Silencing

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recommendations! Published in 2022 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms23105387

Abstract: TRUE gene silencing is an RNA-mediated gene expression control technology and is termed after tRNase ZL-utilizing efficacious gene silencing. In this review, I overview the potentiality of small guide RNA (sgRNA) for TRUE gene silencing… read more here.

Keywords: true gene; gene silencing; pre trna; trnase ... See more keywords

The Regulation of Translation in Alphavirus-Infected Cells

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recommendations! Published in 2018 at "Viruses"

DOI: 10.3390/v10020070

Abstract: Sindbis virus (SINV) contains an RNA genome of positive polarity with two open reading frames (ORFs). The first ORF is translated from the genomic RNA (gRNA), rendering the viral non-structural proteins, whereas the second ORF… read more here.

Keywords: sgrna; translation alphavirus; initiation; sinv sgrna ... See more keywords
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Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

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recommendations! Published in 2022 at "eLife"

DOI: 10.7554/elife.81856

Abstract: CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but… read more here.

Keywords: sgrna libraries; maximizing crispri; crispri; crispri efficacy ... See more keywords