New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference
Sign Up to like & getrecommendations! Published in 2023 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00595
Abstract: CRISPR interference (CRISPRi) screening has been used for identification of target genes related to specific phenotypes using single-molecular guide RNA (sgRNA) libraries. In CRISPRi screening, the sizes of random sgRNA libraries contained with the original… read more here.
Keywords: sgrna libraries; target genes; random sgrna; target gene ... See more keywords
RELATe enables genome-scale engineering in fungal genomics
Sign Up to like & getrecommendations! Published in 2020 at "Science Advances"
DOI: 10.1126/sciadv.abb8783
Abstract: Genomic DNA can be converted into sgRNA libraries for genome-wide screening of fungal factors in blood-brain barrier penetration. CRISPR-Cas9–based screening with single-guide RNA (sgRNA) libraries has emerged as a revolutionary tool for comprehensive analysis of… read more here.
Keywords: enables genome; genome scale; scale engineering; engineering fungal ... See more keywords
Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.81856
Abstract: CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but… read more here.
Keywords: sgrna libraries; maximizing crispri; crispri; crispri efficacy ... See more keywords