Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_13
Abstract: Commercial antibodies raised against phosphotyrosine have been widely used as reagents to detect or isolate tyrosine-phosphorylated proteins from cellular samples. However, these antibodies are costly and are not amenable to in-house production in an academic… read more here.
Keywords: creation phosphotyrosine; sh2; phosphotyrosine superbinders; sh2 domain ... See more keywords
Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain.
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DOI: 10.1007/978-1-4939-6762-9_14
Abstract: Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state,… read more here.
Keywords: state; p59fyn sh2; sh2; state p59fyn ... See more keywords
SH2 Domain Histochemistry.
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DOI: 10.1007/978-1-4939-6762-9_31
Abstract: Among posttranslational modifications, the phosphorylation of tyrosine residues is a key modification in cell signaling. Because of its biological importance, characterization of the cellular state of tyrosine phosphorylation is of great interest. Based on the… read more here.
Keywords: tyrosine phosphorylation; sh2 domain; phosphorylation; tyrosine ... See more keywords
Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes.
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DOI: 10.1007/978-1-4939-6762-9_4
Abstract: Today there exists a rapidly expanding number of sequenced genomes. Cataloging protein interaction domains such as the Src Homology 2 (SH2) domain across these various genomes can be accomplished with ease due to existing algorithms… read more here.
Keywords: tracing sh2; sh2 domains; sh2; domains throughout ... See more keywords
Solution structure of SHIP2 SH2 domain and its interaction with a phosphotyrosine peptide from c-MET.
Sign Up to like & getrecommendations! Published in 2018 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2018.08.012
Abstract: SH2 domain-containing inositol 5-phosphatase 2 (SHIP2) binds with the Y1356-phosphorylated hepatocyte growth factor (HGF) receptor, c-MET, through its SH2 domain, which is essential for the role of SHIP2 in HGF-induced cell scattering and cell spreading.… read more here.
Keywords: sh2; ship2 sh2; structure; sh2 domain ... See more keywords
A regulatory SH2 domain-targeting protein binder effectively inhibits the activity of Bruton's tyrosine kinase and its drug-resistant variants.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.03.006
Abstract: Human Bruton's tyrosine kinase (hBtk) plays a key role in growth and metabolism of B cells, but its dysfunctions cause various B-cell malignancies. Inhibitors targeting the ATP-binding pocket of hBtk have been developed, but they… read more here.
Keywords: protein binder; drug resistant; sh2 domain; activity ... See more keywords
Cellular phosphatase activity of C1-Ten/Tensin2 is controlled by Phosphatidylinositol-3,4,5-triphosphate binding through the C1-Ten/Tensin2 SH2 domain.
Sign Up to like & getrecommendations! Published in 2018 at "Cellular signalling"
DOI: 10.1016/j.cellsig.2018.07.009
Abstract: Regulation of tyrosine phosphorylation on insulin receptor substrate-1 (IRS-1) is essential for insulin signaling. The protein tyrosine phosphatase (PTP) C1-Ten/Tensin2 has been implicated in the regulation of IRS-1, but the molecular basis of this dephosphorylation… read more here.
Keywords: ten tensin2; sh2 domain; tensin2 sh2;
Multimodal Recognition of Diverse Peptides by the C-Terminal SH2 Domain of Phospholipase C-γ1 Protein.
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DOI: 10.1021/acs.biochem.7b00023
Abstract: SH2 domains recognize phosphotyrosine (pY)-containing peptide ligands and play key roles in the regulation of receptor tyrosine kinase pathways. Each SH2 domain has individualized specificity, encoded in the amino acids neighboring the pY, for defined… read more here.
Keywords: protein; terminal sh2; amino acids; recognition ... See more keywords
Optimization of Phosphotyrosine Peptides that Target the SH2 Domain of SOCS1 and Block Substrate Ubiquitination.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00884
Abstract: Suppressor of cytokine signaling 1 (SOCS1) has emerged as a potential therapeutic target in inflammatory and viral diseases. SOCS1 operates via its kinase inhibitory region, Src homology 2 (SH2) domain, and SOCS box to negatively… read more here.
Keywords: domain socs1; sh2 domain; target sh2; domain ... See more keywords
Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening
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DOI: 10.1039/c7ra07445j
Abstract: The Src SH2 domain was synthesized via native chemical ligation of two fragment peptides. The facile protocol was used to prepare the mirror-image SH2 domain (D-Src SH2 domain) and tetramethylrhodamine-labeled SH2 domains. The synthesized SH2… read more here.
Keywords: sh2 domain; mirror image; sh2; src sh2 ... See more keywords
Methanethiosulfonate derivatives as ligands of the STAT3-SH2 domain
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DOI: 10.1080/14756366.2016.1252757
Abstract: Abstract With the aim to discover new STAT3 direct inhibitors, potentially useful as anticancer agents, a set of methanethiosulfonate drug hybrids were synthesized. The in vitro tests showed that all the thiosulfonic compounds were able… read more here.
Keywords: derivatives ligands; sh2 domain; methanethiosulfonate derivatives; stat3 sh2 ... See more keywords