Introduction: History of SH2 Domains and Their Applications.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_1
Abstract: The Src Homology 2 (SH2) domain is the prototypical protein interaction module that lies at the heart of phosphotyrosine signaling. Since its serendipitous discovery, there has been a tremendous advancement in technologies and an array… read more here.
Keywords: sh2 domains; history sh2; sh2; biology ... See more keywords
Expression and Purification of SH2 Domains Using Baculovirus Expression System.
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DOI: 10.1007/978-1-4939-6762-9_11
Abstract: Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble… read more here.
Keywords: sh2 domains; domains using; baculovirus expression; expression system ... See more keywords
Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_4
Abstract: Today there exists a rapidly expanding number of sequenced genomes. Cataloging protein interaction domains such as the Src Homology 2 (SH2) domain across these various genomes can be accomplished with ease due to existing algorithms… read more here.
Keywords: tracing sh2; sh2 domains; sh2; domains throughout ... See more keywords
Proteomic Clustering Analysis of SH2 Domain Datasets.
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DOI: 10.1007/978-1-4939-6762-9_7
Abstract: Proteomic clustering analysis provides a means of identifying relationships and visualizing those relationships in an extremely complex field of study with many interacting parts. With recent high-throughput studies of Src Homology 2 (SH2) domains, many… read more here.
Keywords: sh2 domains; proteomic clustering; analysis sh2; analysis ... See more keywords
Identification and characterization of a large family of superbinding bacterial SH2 domains
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DOI: 10.1038/s41467-018-06943-2
Abstract: Src homology 2 (SH2) domains play a critical role in signal transduction in mammalian cells by binding to phosphorylated Tyr (pTyr). Apart from a few isolated cases in viruses, no functional SH2 domain has been… read more here.
Keywords: sh2 domains; bacterial sh2; sh2; large family ... See more keywords
Dephosphorylation accelerates the dissociation of ZAP70 from the T cell receptor
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2116815119
Abstract: Significance Src homology 2 (SH2) domains are phosphotyrosine binding motifs that play key roles in cellular signaling. There are 110 proteins in the human genome containing SH2 binding domains, of which 10 contain tandem SH2… read more here.
Keywords: cell receptor; zap70; sh2 domains; sh2 ... See more keywords
Phage-Displayed SH2 Domain Libraries: From Ultrasensitive Tyrosine Phosphoproteome Probes to Translational Research.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.over107981
Abstract: Tyrosine phosphorylation is a critical regulator of cell signaling. A large fraction of the tyrosine phosphoproteome, however, remains uncharacterized, largely due to a lack of robust and scalable methods. The Src homology 2 (SH2) domain,… read more here.
Keywords: tyrosine phosphoproteome; sh2 domains; sh2 domain; phosphoproteome ... See more keywords
Identification and characterization of a large family of superbinding bacterial SH2 domains.
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.2210/pdb6e8k/pdb
Abstract: Src homology 2 (SH2) domains play a critical role in signal transduction in mammalian cells by binding to phosphorylated Tyr (pTyr). Apart from a few isolated cases in viruses, no functional SH2 domain has been… read more here.
Keywords: sh2 domains; bacterial sh2; sh2; large family ... See more keywords
The Janus Kinase (JAK) FERM and SH2 Domains: Bringing Specificity to JAK–Receptor Interactions
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Endocrinology"
DOI: 10.3389/fendo.2017.00071
Abstract: The Janus kinases (JAKs) are non-receptor tyrosine kinases essential for signaling in response to cytokines and interferons and thereby control many essential functions in growth, development, and immune regulation. JAKs are unique among tyrosine kinases… read more here.
Keywords: receptor; jak ferm; sh2 domains; ferm sh2 ... See more keywords
High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display
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DOI: 10.7554/elife.82345
Abstract: Tyrosine kinases and SH2 (phosphotyrosine recognition) domains have binding specificities that depend on the amino acid sequence surrounding the target (phospho)tyrosine residue. Although the preferred recognition motifs of many kinases and SH2 domains are known,… read more here.
Keywords: recognition; tyrosine; sh2 domains; tyrosine kinases ... See more keywords