Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducible promoter Ppst
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DOI: 10.1002/btpr.3293
Abstract: Poly‐γ‐glutamic acid (γ‐PGA) is a biopolymer with a wide range of applications, mainly produced using Bacillus strains. The formation and concomitant secretion of γ‐PGA increases the culture broth viscosity, while enzymatic depolymerisation and degradation of… read more here.
Keywords: pga; pga production; shake flasks; knockout ... See more keywords
In vitro liquid culture and optimization of Steinernema jeffreyense using shake flasks
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DOI: 10.1007/s10526-019-09977-7
Abstract: Entomopathogenic nematodes (EPNs) of the families Heterorhabditidae and Steinernematidae are efficient biological control agents against important insect pests. In vitro liquid culture production technology is a key factor in the success of implementing EPNs as… read more here.
Keywords: liquid culture; steinernema jeffreyense; vitro liquid; shake flasks ... See more keywords
CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant
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DOI: 10.1016/j.bej.2019.02.006
Abstract: Abstract Several studies on the impact of cell culture parameters on the profile of host cell protein (HCP) impurities have been carried out in shake flasks. Herein, we explore how transferable the findings and conclusions… read more here.
Keywords: cell protein; host cell; cell; shake flasks ... See more keywords
Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks
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DOI: 10.3389/fbioe.2021.725498
Abstract: Cultivations of mammalian cells are routinely conducted in shake flasks. In contrast to instrumented bioreactors, reliable options for non-invasive, time-resolved monitoring of the culture status in shake flasks are lacking. The Respiration Activity Monitoring Respiration… read more here.
Keywords: time; time resolved; rate; transfer ... See more keywords