Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction
Sign Up to like & getrecommendations! Published in 2025 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.70046
Abstract: In this letter, we put forward the light sheet fluorescence microscopy (LSFM) as a cutting‐edge tool for 3D imaging of whole skeletal muscle, focusing on satellite cells (SCs). SCs represent the resident adult muscle stem… read more here.
Keywords: forced eccentric; satellite cells; sheet fluorescence; fluorescence microscopy ... See more keywords
Monitoring microbial communities using light sheet fluorescence microscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in microbiology"
DOI: 10.1016/j.mib.2017.11.008
Abstract: Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a… read more here.
Keywords: microscopy; fluorescence microscopy; sheet fluorescence; light sheet ... See more keywords
In Situ Visualization of Membrane Fouling Evolution during Ultrafiltration Using Label-Free Hyperspectral Light Sheet Fluorescence Imaging.
Sign Up to like & getrecommendations! Published in 2023 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c08731
Abstract: Profound understanding of fouling behaviors and underlying mechanisms is fundamentally important for fouling control in membrane-based environmental applications. Therefore, it entails novel noninvasive analytical approaches for in situ characterizing the formation and development of membrane… read more here.
Keywords: label free; light sheet; membrane fouling; membrane ... See more keywords
Single-cell light-sheet fluorescence 3D images of tumour-stroma spheroid multicultures
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Data"
DOI: 10.1038/s41597-025-04832-0
Abstract: Spheroids are widely used in oncology for testing drugs, but models composed of a single cell line do not fully capture the complexity of the in vivo tumours targeted by chemotherapy. Developing 3D in vitro… read more here.
Keywords: sheet fluorescence; cell; light sheet; single cell ... See more keywords
Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-08113-8
Abstract: We introduce a concept that enables parallelized three-dimensional imaging throughout large volumes with isotropic 300–350 nm resolution. By staggering high aspect ratio illumination beams laterally and axially within the depth of focus of a digitally scanned… read more here.
Keywords: three dimensional; microscopy; digitally scanned; sheet fluorescence ... See more keywords
Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-63847-2
Abstract: Light-sheet fluorescence microscopy (LSFM) has now become a unique tool in different fields ranging from three-dimensional (3D) tissue imaging to real-time functional imaging of neuronal activities. Nevertheless, obtaining high-quality artifact-free images from large, dense and… read more here.
Keywords: microscopy; fluorescence microscopy; sheet fluorescence; light sheet ... See more keywords
Imaging Enhancement of Light-Sheet Fluorescence Microscopy via Deep Learning
Sign Up to like & getrecommendations! Published in 2019 at "IEEE Photonics Technology Letters"
DOI: 10.1109/lpt.2019.2948030
Abstract: The complementary beam subtraction (CBS) method can reduce the out-of-focus background and improve the axial resolution in light-sheet fluorescence microscopy (LSFM) via double scanning a Bessel and the complementary beams. With the assistance of a… read more here.
Keywords: cbs; light sheet; microscopy; method ... See more keywords
MuscleParseNet: A Novel Framework for Parsing Muscles of Drosophila Larva in Light-Sheet Fluorescence Microscopy Images
Sign Up to like & getrecommendations! Published in 2024 at "IEEE Transactions on Circuits and Systems for Video Technology"
DOI: 10.1109/tcsvt.2023.3338860
Abstract: Accurately parsing (i.e., segmenting and recognizing) muscles of freely-moving animals such as Drosophila larva in light-sheet fluorescence microscopy images is necessary to study the relationship between muscle activity and animal motions. However, this task is… read more here.
Keywords: drosophila larva; sheet fluorescence; muscle; larva light ... See more keywords
Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution
Sign Up to like & getrecommendations! Published in 2019 at "Neurophotonics"
DOI: 10.1117/1.nph.6.1.015005
Abstract: Abstract. The goal of understanding the architecture of neural circuits at the synapse level with a brain-wide perspective has powered the interest in high-speed and large field-of-view volumetric imaging at subcellular resolution. Here, we developed… read more here.
Keywords: resolution; light sheet; microscopy; super resolution ... See more keywords
Enhancing brain image quality with 3D U-net for stripe removal in light sheet fluorescence microscopy
Sign Up to like & getrecommendations! Published in 2024 at "Brain Informatics"
DOI: 10.1186/s40708-024-00236-9
Abstract: Light Sheet Fluorescence Microscopy (LSFM) is increasingly popular in neuroimaging for its ability to capture high-resolution 3D neural data. However, the presence of stripe noise significantly degrades image quality, particularly in complex 3D stripes with… read more here.
Keywords: stripe removal; sheet fluorescence; image; fluorescence microscopy ... See more keywords
Light sheet fluorescence microscopy using axi-symmetric binary phase filters.
Sign Up to like & getrecommendations! Published in 2020 at "Biomedical optics express"
DOI: 10.1364/boe.394841
Abstract: Light sheet fluorescence microscopy (LSFM) has become an indispensable tool in biomedical studies owing to its depth-sectioning capability and low photo-bleaching. The axial resolution in LSFM is determined mainly by the thickness of the illumination… read more here.
Keywords: fluorescence microscopy; light sheet; microscopy; sheet fluorescence ... See more keywords