Monitoring microbial communities using light sheet fluorescence microscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in microbiology"
DOI: 10.1016/j.mib.2017.11.008
Abstract: Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a… read more here.
Keywords: microscopy; fluorescence microscopy; sheet fluorescence; light sheet ... See more keywords
In Situ Visualization of Membrane Fouling Evolution during Ultrafiltration Using Label-Free Hyperspectral Light Sheet Fluorescence Imaging.
Sign Up to like & getrecommendations! Published in 2023 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c08731
Abstract: Profound understanding of fouling behaviors and underlying mechanisms is fundamentally important for fouling control in membrane-based environmental applications. Therefore, it entails novel noninvasive analytical approaches for in situ characterizing the formation and development of membrane… read more here.
Keywords: label free; light sheet; membrane fouling; membrane ... See more keywords
Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-08113-8
Abstract: We introduce a concept that enables parallelized three-dimensional imaging throughout large volumes with isotropic 300–350 nm resolution. By staggering high aspect ratio illumination beams laterally and axially within the depth of focus of a digitally scanned… read more here.
Keywords: three dimensional; microscopy; digitally scanned; sheet fluorescence ... See more keywords
Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-63847-2
Abstract: Light-sheet fluorescence microscopy (LSFM) has now become a unique tool in different fields ranging from three-dimensional (3D) tissue imaging to real-time functional imaging of neuronal activities. Nevertheless, obtaining high-quality artifact-free images from large, dense and… read more here.
Keywords: microscopy; fluorescence microscopy; sheet fluorescence; light sheet ... See more keywords
Imaging Enhancement of Light-Sheet Fluorescence Microscopy via Deep Learning
Sign Up to like & getrecommendations! Published in 2019 at "IEEE Photonics Technology Letters"
DOI: 10.1109/lpt.2019.2948030
Abstract: The complementary beam subtraction (CBS) method can reduce the out-of-focus background and improve the axial resolution in light-sheet fluorescence microscopy (LSFM) via double scanning a Bessel and the complementary beams. With the assistance of a… read more here.
Keywords: cbs; light sheet; microscopy; method ... See more keywords
Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution
Sign Up to like & getrecommendations! Published in 2019 at "Neurophotonics"
DOI: 10.1117/1.nph.6.1.015005
Abstract: Abstract. The goal of understanding the architecture of neural circuits at the synapse level with a brain-wide perspective has powered the interest in high-speed and large field-of-view volumetric imaging at subcellular resolution. Here, we developed… read more here.
Keywords: resolution; light sheet; microscopy; super resolution ... See more keywords
Light sheet fluorescence microscopy using axi-symmetric binary phase filters.
Sign Up to like & getrecommendations! Published in 2020 at "Biomedical optics express"
DOI: 10.1364/boe.394841
Abstract: Light sheet fluorescence microscopy (LSFM) has become an indispensable tool in biomedical studies owing to its depth-sectioning capability and low photo-bleaching. The axial resolution in LSFM is determined mainly by the thickness of the illumination… read more here.
Keywords: fluorescence microscopy; light sheet; microscopy; sheet fluorescence ... See more keywords
Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy.
Sign Up to like & getrecommendations! Published in 2021 at "Biomedical optics express"
DOI: 10.1364/boe.427099
Abstract: Light-sheet fluorescence microscopy (LSFM) is a minimally invasive and high throughput imaging technique ideal for capturing large volumes of tissue with sub-cellular resolution. A fundamental requirement for LSFM is a seamless overlap of the light-sheet… read more here.
Keywords: fluorescence microscopy; light sheet; microscopy; sheet fluorescence ... See more keywords
Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning.
Sign Up to like & getrecommendations! Published in 2022 at "Biomedical optics express"
DOI: 10.1364/boe.447392
Abstract: Light-sheet fluorescence microscopy (LSFM) is a high-speed imaging technique that provides optical sectioning with reduced photodamage. LSFM is routinely used in life sciences for live cell imaging and for capturing large volumes of cleared tissues.… read more here.
Keywords: image; microscopy; sheet fluorescence; illumination ... See more keywords
Three-photon light-sheet fluorescence microscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Optics letters"
DOI: 10.1364/ol.43.005484
Abstract: We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low-photodamage technique for fast volumetric imaging of biological samples. We… read more here.
Keywords: photon; microscopy; sheet fluorescence; light sheet ... See more keywords
A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.1012820
Abstract: Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity. Despite the strengths and growing availability of LSFM systems, no generalized… read more here.
Keywords: light sheet; microscopy; sheet fluorescence; fluorescence microscopy ... See more keywords