Light Sheet Microscopy to Measure Protein Dynamics
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.25451
Abstract: Visualizing protein dynamics is the key to a quantitative understanding of molecular mechanisms in biological systems. Recent developments in fluorescent microscopy techniques allow for novel experimental approaches to study stochastic localization, distribution, and movement of… read more here.
Keywords: protein dynamics; microscopy; measure protein; light sheet ... See more keywords
Scattering‐Based Light‐Sheet Microscopy for Rapid Cellular Imaging of Fresh Tissue
Sign Up to like & getrecommendations! Published in 2020 at "Lasers in Surgery and Medicine"
DOI: 10.1002/lsm.23361
Abstract: Light‐sheet microscopy (LSM) is a novel imaging technology that has been used for imaging fluorescence contrast in basic life science research. In this paper, we have developed a scattering‐based LSM (sLSM) for rapidly imaging the… read more here.
Keywords: microscopy; scattering based; based light; light sheet ... See more keywords
Quantifiable Intravital Light Sheet Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_11
Abstract: Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently… read more here.
Keywords: sheet microscopy; microscopy; intravital light; light sheet ... See more keywords
Image processing tools for petabyte-scale light sheet microscopy data
Sign Up to like & getrecommendations! Published in 2024 at "Nature Methods"
DOI: 10.1038/s41592-024-02475-4
Abstract: Light sheet microscopy is a powerful technique for high-speed 3D imaging of subcellular dynamics and large biological specimens. However, it often generates datasets ranging from hundreds of gigabytes to petabytes in size for a single… read more here.
Keywords: image; processing; sheet microscopy; petabyte scale ... See more keywords
Deconvolution of light sheet microscopy recordings
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-53875-y
Abstract: We developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach… read more here.
Keywords: microscopy; point spread; deconvolution; light sheet ... See more keywords
Light-sheet microscopy with attenuation-compensated propagation-invariant beams
Sign Up to like & getrecommendations! Published in 2018 at "Science Advances"
DOI: 10.1126/sciadv.aar4817
Abstract: Tailoring beams to overcome attenuation allows light-sheet microscopy to image at greater depth with enhanced contrast. Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth… read more here.
Keywords: microscopy; propagation invariant; light sheet; attenuation ... See more keywords
Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes.
Sign Up to like & getrecommendations! Published in 2019 at "Annual review of cell and developmental biology"
DOI: 10.1146/annurev-cellbio-100818-125311
Abstract: The ability to visualize and quantitatively measure dynamic biological processes in vivo and at high spatiotemporal resolution is of fundamental importance to experimental investigations in developmental biology. Light-sheet microscopy is particularly well suited to providing… read more here.
Keywords: microscopy; developmental biology; biology; light sheet ... See more keywords
Microvascular Network Remodeling in the Ischemic Mouse Brain Defined by Light Sheet Microscopy
Sign Up to like & getrecommendations! Published in 2024 at "Arteriosclerosis, Thrombosis, and Vascular Biology"
DOI: 10.1161/atvbaha.123.320339
Abstract: BACKGROUND: Until now, the analysis of microvascular networks in the reperfused ischemic brain has been limited due to tissue transparency challenges. METHODS: Using light sheet microscopy, we assessed microvascular network remodeling in the striatum from… read more here.
Keywords: microvascular network; brain; network remodeling; sheet microscopy ... See more keywords
Design parameters for Airy beams in light-sheet microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Applied optics"
DOI: 10.1364/ao.458343
Abstract: We derive analytical expressions for the length, thickness, and curvature of an Airy light sheet in terms of basic parameters of the cubic phase and the paraxially defined focusing optics that form the beam. The… read more here.
Keywords: light sheet; beam; sheet microscopy; microscopy ... See more keywords
Axial scanning of dual focus to improve light sheet microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Biomedical optics express"
DOI: 10.1364/boe.464292
Abstract: Axially swept light sheet microscopy (ASLM) is an emerging technique that enables isotropic, subcellular resolution imaging with high optical sectioning capability over a large field-of-view (FOV). Due to its versatility across a broad range of… read more here.
Keywords: light sheet; sheet microscopy; microscopy; detection signal ... See more keywords
Large-scale high-throughput 3D culture, imaging, and analysis of cell spheroids using microchip-enhanced light-sheet microscopy.
Sign Up to like & getrecommendations! Published in 2023 at "Biomedical optics express"
DOI: 10.1364/boe.485217
Abstract: Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that notably improves efficiency. However, microchip-enhanced light-sheet microscopy is limited by noticeable aberrations induced by the complex refractive indices in the… read more here.
Keywords: light sheet; sheet microscopy; microscopy; microchip enhanced ... See more keywords