Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8949-2_11
Abstract: Although the presence of antibodies against double-stranded (ds) DNA is considered the serological hallmark of systemic lupus erythematosus (SLE), it is not detected in all SLE patients by routine laboratory tests. Looking at DNA-anti(ds)DNA interaction… read more here.
Keywords: shift assay; mobility shift; electrophoretic mobility; dna ... See more keywords
Thermal shift assay: Strengths and weaknesses of the method to investigate the ligand-induced thermostabilization of soluble guanylyl cyclase.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2019.113065
Abstract: Thermal shift assay is a fluorescence dye based biochemical method to determine the melting point of a protein. It can be used to investigate the ligand-induced stabilization of proteins and helps to increase the likelihood… read more here.
Keywords: shift; shift assay; ligand induced; soluble guanylyl ... See more keywords
Matrix Thermal Shift Assay for Fast Construction of Multidimensional Ligand-Target Space.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04627
Abstract: Existing thermal shift-based mass spectrometry approaches are able to identify target proteins without chemical modification of the ligand, but they are suffering from complicated workflows with limited throughput. Herein, we present a new thermal shift-based… read more here.
Keywords: ligand; shift assay; matrix thermal; shift ... See more keywords
High-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00760
Abstract: Cellular thermal shift assay (CETSA) is a valuable method to confirm target engagement within a complex cellular environment, by detecting changes in a protein's thermal stability upon ligand binding. The classical CETSA method measures changes… read more here.
Keywords: using acoustic; thermal shift; shift assay; high throughput ... See more keywords
Determining direct binders of the Androgen Receptor using a high-throughput Cellular Thermal Shift Assay
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18650-x
Abstract: Androgen Receptor (AR) is a key driver in prostate cancer. Direct targeting of AR has valuable therapeutic potential. However, the lack of disease relevant cellular methodologies capable of discriminating between inhibitors that directly bind AR… read more here.
Keywords: direct binders; shift assay; cellular thermal; receptor ... See more keywords
Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118220119
Abstract: Significance Chemical genetics, which investigates biological processes using small molecules, is gaining interest in plant research. However, a major challenge is to uncover the mode of action of the small molecules. Here, we applied the… read more here.
Keywords: shift assay; auxin; cellular thermal; thermal shift ... See more keywords
TSA-CRAFT: A Free Software for Automatic and Robust Thermal Shift Assay Data Analysis
Sign Up to like & getrecommendations! Published in 2019 at "Slas Discovery"
DOI: 10.1177/2472555218823547
Abstract: Thermal shift assay (TSA) is an increasingly popular technique used for identifying protein stabilizing conditions or interacting ligands in X-ray crystallography and drug discovery applications. Although the setting up and running of TSA reactions is… read more here.
Keywords: tsa craft; shift assay; analysis; tsa ... See more keywords