Articles with "shoot tips" as a keyword



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Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.

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Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0783-1_35

Abstract: Cryopreservation of shoot tips facilitates long-term storage of plant genetic resources which can otherwise only be propagated vegetatively. The vitrification approach using the cryoprotectant plant vitrification solution 3 (PVS3, 50% sucrose and 50% glycerol) is… read more here.

Keywords: plant; potato mint; cryopreservation; mint garlic ... See more keywords
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Recovery of cryopreserved silver birch shoot tips is affected by the pre-freezing age of the cultures and ammonium substitution

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Published in 2019 at "Plant Cell Reports"

DOI: 10.1007/s002990100336

Abstract: Abstract. The aim of the present study was to improve the cryopreservation protocol for silver birch (Betula pendula Roth) in vitro shoot tips. The recovery of shoot tips derived from young cultures (shoot tips excised… read more here.

Keywords: shoot tips; recovery; substitution; silver birch ... See more keywords
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Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

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Published in 2017 at "Acta Physiologiae Plantarum"

DOI: 10.1007/s11738-017-2593-4

Abstract: Cryopreservation is widely applied to many economically important species excluding chimera plants which are problematic for long-term conservation. Their storage problems can be circumvented only by cryopreserving meristems. This study looked at the morphogenetic response… read more here.

Keywords: cryopreservation; shoot tips; shoot; morphogenetic response ... See more keywords
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Osmo- and cryotherapy of sugarcane (Saccharum spp. L.) shoot-tips infected with sugarcane mosaic virus (SCMV).

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Published in 2020 at "Virusdisease"

DOI: 10.1007/s13337-020-00636-5

Abstract: In this work, we investigated the effect of different osmoprotective treatments and of cryopreservation using a droplet-vitrification (D-V) protocol to eliminate sugarcane mosaic virus (SCMV) of shoot-tips excised from in vitro propagated infected plantlets. Shoot-tips… read more here.

Keywords: mosaic virus; sugarcane; sugarcane mosaic; scmv ... See more keywords
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Virus infection reduces shoot proliferation of in vitro stock cultures and ability of cryopreserved shoot tips to regenerate into normal shoots in 'Gala' apple (Malus × domestica).

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Published in 2018 at "Cryobiology"

DOI: 10.1016/j.cryobiol.2018.08.002

Abstract: Plant cryopreservation has provide secure back-ups of germplasm collections of vegetatively propagated crops. Often, recovery levels vary among laboratories when the same cryogenic procedures are used for the same genotypes. The present study investigated the… read more here.

Keywords: stock cultures; shoot proliferation; proliferation vitro; vitro stock ... See more keywords
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The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips

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Published in 2020 at "Journal of Experimental Botany"

DOI: 10.1093/jxb/eraa224

Abstract: Storage of meristematic tissue at ultra-low temperatures offers a mean to maintain valuable genetic resources from vegetatively reproduced plants. To reveal the biology underlying cryo-stress, shoot tips of the model plant Arabidopsis thaliana were subjected… read more here.

Keywords: factor wrky22; transcription factor; stress; shoot tips ... See more keywords
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Critical vitrification steps towards survival of Garcinia hombroniana (Clusiaceae) shoot tips after cryopreservation

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Published in 2018 at "Revista De Biologia Tropical"

DOI: 10.15517/rbt.v66i3.31483

Abstract: A cryopreservation protocol was developed for in vitro shoot tips of Garcinia hombroniana using the vitrification technique. Four critical steps in the technique were investigated, namely preculture, loading, dehydration with Plant Vitrification Solution 2 (PVS2),… read more here.

Keywords: tips cryopreservation; vitrification; cryopreservation; garcinia hombroniana ... See more keywords
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Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

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Published in 2023 at "Horticulturae"

DOI: 10.3390/horticulturae9050559

Abstract: Juglans regia L. is a crucial species as a forest tree and for its nutritional and medicinal values. It is also included in the list of endangered species in Albania and thus, there is a… read more here.

Keywords: regeneration; conservation; synthetic seeds; shoot tips ... See more keywords
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An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte

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Published in 2020 at "Plants"

DOI: 10.3390/plants9020235

Abstract: Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation… read more here.

Keywords: sporophyte; selaginella martensii; shoot tip; shoot tips ... See more keywords