Articles with "shotgun sequencing" as a keyword



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From Whole-Genome Shotgun Sequencing to Viral Community Profiling: The ViromeScan Tool.

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Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7683-6_14

Abstract: ViromeScan is an innovative metagenomic analysis tool that allows the viral community characterization in terms of taxonomy from raw data of metagenomics sequencing. It efficiently denoises samples from reads of other microorganisms. Users can adopt… read more here.

Keywords: whole genome; genome shotgun; tool; shotgun sequencing ... See more keywords

Whole genome shotgun sequencing of POPs degrading bacterial community dwelling tannery effluents and petrol contaminated soil.

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Published in 2020 at "Microbiological research"

DOI: 10.1016/j.micres.2020.126504

Abstract: The present study involved identification of genes which are present in the genome of native bacteria to make them effective tools for bioremediation of persistent organic pollutants (POPs). During this study, forty-one POPs (naphthalene, toluene… read more here.

Keywords: petrol; genome shotgun; whole genome; shotgun sequencing ... See more keywords

16S rRNA and metagenomic shotgun sequencing data revealed consistent patterns of gut microbiome signature in pediatric ulcerative colitis

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Published in 2022 at "Scientific Reports"

DOI: 10.1038/s41598-022-07995-7

Abstract: Dysbiosis of human gut microbiota has been reported in association with ulcerative colitis (UC) in both children and adults using either 16S rRNA gene or shotgun sequencing data. However, these studies used either 16S rRNA… read more here.

Keywords: rrna metagenomic; 16s rrna; sequencing data; metagenomic shotgun ... See more keywords

Shotgun sequencing of clinical biofilm following scanning electron microscopy identifies bacterial community composition

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Published in 2019 at "Pathogens and Disease"

DOI: 10.1093/femspd/ftz013

Abstract: &NA; Bacterial biofilm infections often involve aggregates of bacteria heterogeneously distributed throughout a tissue or on a surface (such as an implanted medical device). Identification of a biofilm infection requires direct visualization via microscopy, followed… read more here.

Keywords: microscopy; community; shotgun sequencing; electron microscopy ... See more keywords
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Performance of amplicon and shotgun sequencing for accurate biomass estimation in invertebrate community samples

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Published in 2018 at "Molecular Ecology Resources"

DOI: 10.1111/1755-0998.12888

Abstract: New applications of DNA and RNA sequencing are expanding the field of biodiversity discovery and ecological monitoring, yet questions remain regarding precision and efficiency. Due to primer bias, the ability of metabarcoding to accurately depict… read more here.

Keywords: amplicon shotgun; performance amplicon; biomass; shotgun sequencing ... See more keywords

Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis

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Published in 2017 at "Journal of Clinical Microbiology"

DOI: 10.1128/jcm.02402-16

Abstract: ABSTRACT Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. Depending on the application, background DNA from WGA kits… read more here.

Keywords: whole genome; sequencing infection; shotgun sequencing; metagenomic shotgun ... See more keywords

Using Shotgun Sequencing to Describe the Changes Induced by In-Feed Zinc Oxide and Apramycin in the Microbiomes of Pigs One Week Postweaning

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Published in 2022 at "Microbiology Spectrum"

DOI: 10.1128/spectrum.01597-22

Abstract: Weaning is a critical period for piglets, during which potentially harmful bacteria such as Escherichia coli can increase in abundance in the intestine, creating digestive problems and diarrhea. In-feed antibiotics, the most frequent administration route… read more here.

Keywords: shotgun sequencing; feed; abundance; using shotgun ... See more keywords

Metagenomic shotgun sequencing to identify specific human gut microbes associated with immune checkpoint therapy efficacy in melanoma patients.

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Published in 2017 at "Journal of Clinical Oncology"

DOI: 10.1200/jco.2017.35.15_suppl.9516

Abstract: 9516Background: Immune checkpoint inhibitor therapy, ICT, achieves durable remissions in 30-50% of patients (pts) with metastatic melanoma (Larkin et al. NEJM 2015). It is still unclear what host f... read more here.

Keywords: melanoma; immune checkpoint; shotgun sequencing; metagenomic shotgun ... See more keywords

Whole Genome Shotgun Sequencing-Based Insights into the Benzene and Xylene Degrading Potentials of Bacteria

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Published in 2025 at "Polish Journal of Microbiology"

DOI: 10.33073/pjm-2025-020

Abstract: Abstract Due to their hazardous effects on human health and air quality, benzene and xylene constitute the primary pollutants. Coupling the physicochemical strategies with bacterial bioremediation is an emerging mode of decontamination. Considering the limited… read more here.

Keywords: benzene xylene; whole genome; genome shotgun; xylene ... See more keywords

No evidence on infectious DNA-based agents in pediatric acute lymphoblastic leukemia using whole metagenome shotgun sequencing

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Published in 2024 at "Frontiers in Cellular and Infection Microbiology"

DOI: 10.3389/fcimb.2024.1355787

Abstract: The etiology of pediatric acute lymphatic leukemia (ALL) is still unclear. Whole-metagenome shotgun sequencing of bone marrow samples in patients with treatment-naïve ALL (n=6) was performed for untargeted investigation of bacterial and viral DNA. The… read more here.

Keywords: pediatric acute; leukemia; shotgun sequencing; metagenome shotgun ... See more keywords

Metagenomic shotgun sequencing and metabolomic profiling identify specific human gut microbiota associated with diabetic retinopathy in patients with type 2 diabetes

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Published in 2022 at "Frontiers in Immunology"

DOI: 10.3389/fimmu.2022.943325

Abstract: Background Diabetic retinopathy (DR) is a common microvascular complication of diabetes mellitus (DM) and is one of the leading causes of blindness among DM patients. However, the molecular mechanism involving DR remains unclear. Methods A… read more here.

Keywords: gut microbiota; shotgun sequencing; metabolomic profiling; group ... See more keywords