Cytoplasmic expression of EGFR shRNA using a modified T7 autogene-based hybrid mRNA/DNA system induces long-term EGFR silencing and prolongs antitumor effects.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical pharmacology"
DOI: 10.1016/j.bcp.2019.113735
Abstract: Unusual activation or overexpression of epidermal growth factor receptor (EGFR) has been found in various cancers, and therefore down-regulation of EGFR expression is recognized as a promising strategy for cancer treatment. For decades, RNAi has… read more here.
Keywords: egfr; autogene based; system; shrna ... See more keywords
Intercalation of shRNA-plasmid in Mg–Al layered double hydroxide nanoparticles and its cellular internalization for possible treatment of neurodegenerative diseases
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Drug Delivery Science and Technology"
DOI: 10.1016/j.jddst.2019.05.008
Abstract: Abstract In the present work, nanoconjugates of shRNA-plasmid and a non-viral nanoceramic vector, e.g., Mg–Al layered double hydroxide (Mg-Al LDH), were synthesized and intercalated. Subsequently, these particles with an average size of 40–60 nm, were transfected… read more here.
Keywords: shrna plasmid; layered double; shrna; double hydroxide ... See more keywords
Injectable in situ forming hydrogel gene depot to improve the therapeutic effect of STAT3 shRNA.
Sign Up to like & getrecommendations! Published in 2021 at "Biomaterials science"
DOI: 10.1039/d1bm00624j
Abstract: Down-regulation of the signal transducer and activity of transcription 3 (Stat3) plays a crucial role in suppression of many solid tumors. Intratumoral injection of a gene carrier applying Stat3-small hairpin RNA (St3-shRNA) is a potential… read more here.
Keywords: st3 shrna; shrna; gene; shrna nh2 ... See more keywords
Targeted delivery of irinotecan and SLP2 shRNA with GRP-conjugated magnetic graphene oxide for glioblastoma treatment.
Sign Up to like & getrecommendations! Published in 2022 at "Biomaterials science"
DOI: 10.1039/d2bm00420h
Abstract: Magnetic nanoparticles (MNPs) are useful for magnetic targeted drug delivery while ligand-mediated active targeting is another common delivery strategy for cancer therapy. In this work, we intend to prepare magnetic graphene oxide (mGO) by chemical… read more here.
Keywords: shrna; glioblastoma; magnetic graphene; treatment ... See more keywords
Development of a novel method for rapid cloning of shRNA vectors, which successfully knocked down CD44 in mesenchymal triple-negative breast cancer cells
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Communications"
DOI: 10.1186/s40880-018-0327-7
Abstract: © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided… read more here.
Keywords: vector; novel method; method; shrna ... See more keywords
Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0191570
Abstract: In the search for novel therapeutic targets, RNA interference screening has become a valuable tool. High-throughput technologies are now broadly accessible but their assay development from baseline remains resource-intensive and challenging. Focusing on this assay… read more here.
Keywords: analysis; target discovery; target; shrna ... See more keywords
Silencing TLR4 using an ultrasound-targeted microbubble destruction-based shRNA system reduces ischemia-induced seizures in hyperglycemic rats
Sign Up to like & getrecommendations! Published in 2022 at "Open Life Sciences"
DOI: 10.1515/biol-2022-0526
Abstract: Abstract The toll-like receptor 4 (TLR4) pathway is involved in seizures. We investigated whether ultrasound-targeted microbubble destruction (UTMD)-mediated delivery of short hairpin RNA (shRNA) targeting the TLR4 gene (shRNA-TLR4) can reduce ischemia-induced seizures in rats… read more here.
Keywords: shrna tlr4; shrna; ischemia induced; ultrasound targeted ... See more keywords
Preparation of DSPE-PEG-cRGD Modified Cationic Liposomes for Delivery of OC-2 shRNA and The Antitumor Effects on Breast Cancer
Sign Up to like & getrecommendations! Published in 2022 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics14102157
Abstract: Cationic liposome delivery of interfering RNA (shRNA) plays an important role in tumor therapy. The cyclic Arg-Gly-Asp (cRGD) modified cationic liposomes (cRGD-CL) were designed for targeted delivery of ONECUT2 (OC-2) shRNA (pshOC-2) to breast cancer… read more here.
Keywords: shrna; crgd; breast cancer; crgd modified ... See more keywords
Downregulation of mouse CCR3 by lentiviral shRNA inhibits proliferation and induces apoptosis of mouse eosinophils
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Medicine Reports"
DOI: 10.3892/mmr.2016.6085
Abstract: RNA interference has been considered as an effective gene silencing method in basic and preclinical investigations. The aims of the present study were to construct a lentiviral vector expressing a short hairpin RNA (shRNA) targeting… read more here.
Keywords: proliferation; shrna; mouse eosinophils; apoptosis mouse ... See more keywords
Effects of silenced PAR-2 on cell proliferation, invasion and metastasis of esophageal cancer
Sign Up to like & getrecommendations! Published in 2017 at "Oncology Letters"
DOI: 10.3892/ol.2017.6711
Abstract: The present study aimed to investigate the effect of protease-activated receptor 2 (PAR-2) on cell proliferation, invasion and metastasis in the esophageal EC109 cell line. Two short hairpin RNA (shRNA) expression plasmids were constructed based… read more here.
Keywords: esophageal cancer; par; invasion; proliferation ... See more keywords
Lentiviral delivery of CTLA-4 shRNA improves the expansion of cytokine-induced killer cells and enhances cytotoxic activity in vitro
Sign Up to like & getrecommendations! Published in 2018 at "Oncology Letters"
DOI: 10.3892/ol.2017.7376
Abstract: Cytokine-induced killer (CIK) cells are in vitro-expanded cells harboring potent toxicity against tumor cells. Recently, it was identified that the cytotoxicity and proliferation of CIK cells are restricted by a prolonged CIK cell culture period.… read more here.
Keywords: ctla; cik; shrna; expression ... See more keywords