Transient CHO expression platform for robust antibody production and its enhanced N‐glycan sialylation on therapeutic glycoproteins
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2724
Abstract: Large‐scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery. In this study we demonstrate transient expression in a Chinese hamster ovary (CHO)… read more here.
Keywords: cho expression; production; sialylation; therapeutic glycoproteins ... See more keywords
Enzymatic Sialylation of Synthetic Multivalent Scaffolds: From 3'-Sialyllactose Glycomacromolecules to Novel Neoglycosides.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200358
Abstract: Sialoglycans play a key role in many biological recognition processes and sialylated conjugates of various types have successfully been applied, e.g., as antivirals or in anti-tumor therapy. A key feature for high affinity binding of… read more here.
Keywords: sialylation; enzymatic sialylation; multivalent scaffolds; synthetic multivalent ... See more keywords
Increased Transferrin Sialylation Predicts Phenoconversion in Isolated REM Sleep Behavior Disorder
Sign Up to like & getrecommendations! Published in 2022 at "Movement Disorders"
DOI: 10.1002/mds.28942
Abstract: Sialic acid–protein interactions are involved in regulating central nervous system immunity; therefore, derangements in sialylation could be involved in neurodegeneration. read more here.
Keywords: transferrin sialylation; sialylation; increased transferrin; phenoconversion isolated ... See more keywords
Role of sialylation of N-linked glycans in prion pathogenesis.
Sign Up to like & getrecommendations! Published in 2022 at "Cell and tissue research"
DOI: 10.1007/s00441-022-03584-2
Abstract: Mammalian prion or PrPSc is a proteinaceous infectious agent that consists of a misfolded, self-replicating state of the prion protein or PrPC. PrPC and PrPSc are posttranslationally modified with N-linked glycans, which are sialylated at… read more here.
Keywords: role sialylation; linked glycans; strain; prion ... See more keywords
Terminal α2,6-sialylation of epidermal growth factor receptor modulates antibody therapy response of colorectal cancer cells.
Sign Up to like & getrecommendations! Published in 2021 at "Cellular oncology"
DOI: 10.1007/s13402-021-00606-z
Abstract: BACKGROUND The epidermal growth factor receptor (EGFR) is a key protein involved in cancer development. Monoclonal antibodies targeting EGFR are approved for the treatment of metastatic colorectal cancer (CRC). Despite the beneficial clinical effects observed… read more here.
Keywords: therapy; terminal sialylation; cancer; sialylation ... See more keywords
Sialylation status and mechanical properties of THP-1 macrophages upon LPS stimulation.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.08.089
Abstract: Cell surface receptors are the key contributors of macrophage function. Most macrophage cell surface receptors are glycoproteins with sialic acids at the terminal of their glycans. It is well recognized that lipopolysaccharide (LPS) induces cell… read more here.
Keywords: sialylation status; macrophages upon; macrophage; sialylation ... See more keywords
Native Ion Mobility-Mass Spectrometry-Enabled Fast Structural Interrogation of Labile Protein Surface Modifications at the Intact Protein Level.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04503
Abstract: Protein sialylation has been closely linked to many diseases including Alzheimer's disease (AD). It is also broadly implicated in therapeutics operating in a pattern-dependent (e.g., Neu5Ac vs Neu5Gc) manner. However, how the sialylation pattern affects… read more here.
Keywords: mass spectrometry; sialylation; fast structural; native ion ... See more keywords
α2,6-Sialylation Is Upregulated in Severe COVID-19, Implicating the Complement Cascade
Sign Up to like & getrecommendations! Published in 2022 at "ACS Infectious Diseases"
DOI: 10.1021/acsinfecdis.2c00421
Abstract: Better understanding of the molecular mechanisms underlying COVID-19 severity is desperately needed in current times. Although hyper-inflammation drives severe COVID-19, precise mechanisms triggering this cascade and what role glycosylation might play therein are unknown. Here… read more here.
Keywords: severe covid; sialylation; sialylation upregulated; complement cascade ... See more keywords
Sialylation of immunoglobulin E is a determinant of allergic pathogenicity
Sign Up to like & getrecommendations! Published in 2020 at "Nature"
DOI: 10.1038/s41586-020-2311-z
Abstract: Approximately one-third of the world’s population suffers from allergies 1 . Exposure to allergens crosslinks immunoglobulin E (IgE) antibodies that are bound to mast cells and basophils, triggering the release of inflammatory mediators, including histamine… read more here.
Keywords: non atopic; allergic disease; immunoglobulin; individuals peanut ... See more keywords
Patients with IgG1-anti-red blood cell autoantibodies show aberrant Fc-glycosylation
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-08654-y
Abstract: Autoimmune hemolytic anemia (AIHA) is a potentially severe disease in which red blood cells (RBC) are destroyed by IgG anti-RBC autoantibodies which can lead to hemolysis. We recently found IgG Fc-glycosylation towards platelet and RBC… read more here.
Keywords: red blood; galactosylation; glycosylation; sialylation ... See more keywords
Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-25580-9
Abstract: Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life. N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid. Here, we introduce a new approach… read more here.
Keywords: cho cells; inhibition; sialylation recombinant; poly lacnac ... See more keywords