HighâThroughput Screening of Signal Peptide Library with Novel Fluorescent Probe
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202100523
Abstract: Nitrile hydratase (NHase) is an excellent biocatalyst for the synthesis of amide compounds and is composed of two heterologous subunits. However, the secretory expression of NHase has been difficult to achieve because of its complex… read more here.
Keywords: peptide library; fluorescent probe; throughput screening; high throughput ... See more keywords
18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Analysis
Sign Up to like & getrecommendations! Published in 2021 at "Current Microbiology"
DOI: 10.1007/s00284-020-02309-4
Abstract: GP64 is the key membrane fusion protein of Group I baculovirus, and while the Bombyx mori nucleopolyhedrovirus (BmNPV) GP64 contains a longer n-region (18 amino acid) of the signal peptide than does the Autographa californica… read more here.
Keywords: protein; bombyx mori; gp64; signal peptide ... See more keywords
Improved secretion of recombinant human IL-25 in HEK293 cells using a signal peptide-pro-peptide domain derived from Trypsin-1.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology letters"
DOI: 10.1007/s10529-020-03072-z
Abstract: OBJECTIVE To compare the effects of human Trypsin-1 signal peptide and pro-peptide on the expression and secretion efficiency of human Interleukin-25 from mammalian cells. RESULTS The signal peptide and combined signal peptide-pro-peptide sequence of human… read more here.
Keywords: pro peptide; improved secretion; secretion; signal peptide ... See more keywords
Evaluation of Different Signal Peptides Using Bioinformatics Tools to Express Recombinant Erythropoietin in Mammalian Cells
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Peptide Research and Therapeutics"
DOI: 10.1007/s10989-018-9746-1
Abstract: Nowadays, engineered mammalian cell lines such as CHO and HEK-293 are extensively used to express recombinant proteins which require post translation modifications. Post translation modifications completely depend on presence of an appropriate signal peptide in… read more here.
Keywords: signal peptides; recombinant erythropoietin; signal peptide; express recombinant ... See more keywords
Production of 12-hydroxy dodecanoic acid methyl ester using a signal peptide sequence-optimized transporter AlkL and a novel monooxygenase.
Sign Up to like & getrecommendations! Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2019.121812
Abstract: In this study, a signal peptide of AlkL was replaced with other signal peptides to improve the soluble expression and thereby facilitate the transport of dodecanoic acid methyl ester (DAME) substrate into the E. coli.… read more here.
Keywords: monooxygenase; dodecanoic acid; ester; signal peptide ... See more keywords
A thermostable chitinase from the antagonistic Chromobacterium violaceum that inhibits the development of phytopathogenic fungi.
Sign Up to like & getrecommendations! Published in 2019 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.03.009
Abstract: The biocontrol activity of some soil strains of Chromobacterium sp. against pathogenic fungi has been attributed to secreted chitinases. The aim of this work was to characterize biochemically a recombinant chitinase (CvChi47) from C. violaceum… read more here.
Keywords: phytopathogenic fungi; cvchi47; chitinase; thermostable chitinase ... See more keywords
Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2017.10.014
Abstract: A total of 97 amino acids, considered as the signal peptide and transmembrane segments were removed from 205y lipase gene using polymerase chain reaction technique that abolished the low activity of this enzyme. The mature… read more here.
Keywords: peptide transmembrane; lipase; signal peptide; transmembrane segments ... See more keywords
Protein S-Leu17Pro disrupts the hydrophobicity of its signal peptide causing a proteasome-dependent degradation.
Sign Up to like & getrecommendations! Published in 2021 at "Thrombosis research"
DOI: 10.1016/j.thromres.2021.12.014
Abstract: INTRODUCTION Protein S is a vitamin K-dependent glycoprotein with important anticoagulant, fibrinolytic, anti-inflammatory, anti-apoptotic, and cytoprotective functions. Congenital protein S deficiency is an autosomal dominant thrombophilia due to protein S gene (PROS1) variations. Our group… read more here.
Keywords: signal peptide; recombinant protein; protein deficiency; protein ... See more keywords
Introducing a cleavable signal peptide enhances the packaging efficiency of lentiviral vectors pseudotyped with Japanese encephalitis virus envelope proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Virus research"
DOI: 10.1016/j.virusres.2016.12.007
Abstract: Research into the properties of Japanese encephalitis virus (JEV) has been facilitated by use of pseudotyped viruses. The signal peptide is a key determinant for membrane targeting and membrane insertion, which could affect packaging of… read more here.
Keywords: pseudotyped jev; jev based; signal peptide; lentiviral vectors ... See more keywords
Discovery of the First Potent, Selective, and Orally Bioavailable Signal Peptide Peptidase-Like 2a (SPPL2a) Inhibitor Displaying Pronounced Immunomodulatory Effects In Vivo.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.7b01371
Abstract: Signal peptide peptidase-like 2a (SPPL2a) is an aspartic intramembrane protease which has recently been shown to play an important role in the development and function of antigen presenting cells such as B lymphocytes and dendritic… read more here.
Keywords: sppl2a; signal peptide; peptidase like; discovery first ... See more keywords
SignalP 5.0 improves signal peptide predictions using deep neural networks
Sign Up to like & getrecommendations! Published in 2019 at "Nature Biotechnology"
DOI: 10.1038/s41587-019-0036-z
Abstract: Signal peptides (SPs) are short amino acid sequences in the amino terminus of many newly synthesized proteins that target proteins into, or across, membranes. Bioinformatic tools can predict SPs from amino acid sequences, but most… read more here.
Keywords: deep neural; signal peptides; signal peptide; signalp improves ... See more keywords