Evaluation of Different Signal Peptides Using Bioinformatics Tools to Express Recombinant Erythropoietin in Mammalian Cells
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Peptide Research and Therapeutics"
DOI: 10.1007/s10989-018-9746-1
Abstract: Nowadays, engineered mammalian cell lines such as CHO and HEK-293 are extensively used to express recombinant proteins which require post translation modifications. Post translation modifications completely depend on presence of an appropriate signal peptide in… read more here.
Keywords: signal peptides; recombinant erythropoietin; signal peptide; express recombinant ... See more keywords
Stringency of bacterial prolipoprotein signal peptidase (LspA) in recognition of signal peptides - Structure-function correlation.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.05.069
Abstract: Bacterial lipid modification of proteins is an essential post-translational event committed by Phosphatidylglycerol: prolipoprotein diacylglyceryl transferase (Lgt) by catalysing diacyglyceryl transfer from Phosphatidylglycerol to cysteine present in the characteristic 'lipobox' ([LVI] (-3) [ASTVI] (-2) [GAS]… read more here.
Keywords: prolipoprotein signal; signal peptidase; signal peptides; peptidase lspa ... See more keywords
Widespread pre-translational regulation of the inclusion of signal peptides in human proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Genomics"
DOI: 10.1016/j.ygeno.2017.01.001
Abstract: Signal peptides (SP) are cleavable N-terminal protein motifs used co-translationally for entry of nascent polypeptides into the secretory pathway. Their co-translational cleavage prevents their extensive post-translational regulation and flexibility in their usage is made possible… read more here.
Keywords: regulation inclusion; inclusion; signal peptides; pre translational ... See more keywords
Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.8b04183
Abstract: Bacillus subtilis is widely used for large-scale industrial production of heterologous proteins. Because of its high intrinsic secretory capacity, it can efficiently secrete proteins into the culture supernatant via the general Sec-type secretion pathway. In… read more here.
Keywords: signal peptides; bacillus subtilis; production heterologous; heterologous proteins ... See more keywords
SignalP 5.0 improves signal peptide predictions using deep neural networks
Sign Up to like & getrecommendations! Published in 2019 at "Nature Biotechnology"
DOI: 10.1038/s41587-019-0036-z
Abstract: Signal peptides (SPs) are short amino acid sequences in the amino terminus of many newly synthesized proteins that target proteins into, or across, membranes. Bioinformatic tools can predict SPs from amino acid sequences, but most… read more here.
Keywords: deep neural; signal peptides; signal peptide; signalp improves ... See more keywords
SignalP 6.0 predicts all five types of signal peptides using protein language models
Sign Up to like & getrecommendations! Published in 2022 at "Nature Biotechnology"
DOI: 10.1038/s41587-021-01156-3
Abstract: Signal peptides (SPs) are short amino acid sequences that control protein secretion and translocation in all living organisms. SPs can be predicted from sequence data, but existing algorithms are unable to detect all known types… read more here.
Keywords: predicts five; signalp predicts; five types; peptides using ... See more keywords
In silico high throughput mutagenesis and screening of signal peptides to mitigate N-terminal heterogeneity of recombinant monoclonal antibodies
Sign Up to like & getrecommendations! Published in 2022 at "mAbs"
DOI: 10.1080/19420862.2022.2044977
Abstract: ABSTRACT N-terminal heterogeneity resulting from non-uniform signal peptide (SP) cleavage can potentially affect biologics property attributes and result in extended product development timelines. Few studies are available on engineering SPs systematically to address miscleavage issues.… read more here.
Keywords: terminal heterogeneity; signal peptides; high throughput; miscleavage ... See more keywords
Evolutionary Interplay between Symbiotic Relationships and Patterns of Signal Peptide Gain and Loss
Sign Up to like & getrecommendations! Published in 2018 at "Genome Biology and Evolution"
DOI: 10.1093/gbe/evy049
Abstract: Abstract Can orthologous proteins differ in terms of their ability to be secreted? To answer this question, we investigated the distribution of signal peptides within the orthologous groups of Enterobacterales. Parsimony analysis and sequence comparisons… read more here.
Keywords: signal peptides; gain loss; signal peptide; peptide gain ... See more keywords
Signal Peptide Hydrophobicity Modulates Interaction with the Twin-Arginine Translocase
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DOI: 10.1128/mbio.00909-17
Abstract: ABSTRACT The general secretory pathway (Sec) and twin-arginine translocase (Tat) operate in parallel to export proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of plant chloroplasts. Substrates are targeted to their respective… read more here.
Keywords: signal peptides; signal peptide; tat pathway; twin arginine ... See more keywords
Stable Signal Peptides and the Response to Secretion Stress in Staphylococcus aureus
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DOI: 10.1128/mbio.01507-17
Abstract: ABSTRACT Protein secretion is essential, but how it is managed is poorly understood. In bacteria, most secreted proteins require release from the outer surface of the cytoplasmic membrane by type I signal peptidase (SPase), which… read more here.
Keywords: staphylococcus aureus; secretion; spase; signal peptides ... See more keywords
Bioinformatics analysis of various signal peptides for periplasmic expression of parathyroid hormone in E.coli
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Medicine and Life"
DOI: 10.25122/jml-2018-0049
Abstract: Hypoparathyroidism is a rare endocrine disease which is characterized by the deficiency of serum calcium levels. RhPTH is prescribed as a therapy for the management of refractory hypoparathyroidism. The aim of this study is to… read more here.
Keywords: signal peptides; analysis various; bioinformatics analysis; coli ... See more keywords