Massive parallel insertion site sequencing of an arrayed Sinorhizobium meliloti signature-tagged mini-Tn 5 transposon mutant library.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2017.02.019
Abstract: Transposon mutagenesis in conjunction with identification of genomic transposon insertion sites is a powerful tool for gene function studies. We have implemented a protocol for parallel determination of transposon insertion sites by Illumina sequencing involving… read more here.
Keywords: mutant library; insertion; transposon; signature tagged ... See more keywords
Use of signature-tagged mutagenesis to identify genes associated with colonization of sheep by E. coli O157:H7.
Sign Up to like & getrecommendations! Published in 2017 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2017.01.031
Abstract: Outbreaks of Escherichia coli O157:H7 in the United States due to contaminated foods are a public health issue and a continuing problem. The major reservoir for these organisms is the gastrointestinal tract of ruminants where… read more here.
Keywords: coli o157; colonization; tagged mutagenesis; o157 ... See more keywords
Signature-tagged mutagenesis screening revealed a novel smooth-to-rough transition determinant of Salmonella enterica serovar Enteritidis
Sign Up to like & getrecommendations! Published in 2017 at "BMC Microbiology"
DOI: 10.1186/s12866-017-0951-4
Abstract: BackgroundSalmonella enterica serovar Enteritidis (S. Enteritidis) has emerged as one of the most important food-borne pathogens for humans. Lipopolysaccharide (LPS), as a component of the outer membrane, is responsible for the virulence and smooth-to-rough transition… read more here.
Keywords: rough transition; smooth rough; signature tagged; enteritidis ... See more keywords
Identification of in vivo Essential Genes of Vibrio vulnificus for Establishment of Wound Infection by Signature-Tagged Mutagenesis
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.00123
Abstract: Vibrio vulnificus can cause severe necrotic lesions within a short time. Recently, it has been reported that the numbers of wound infection cases in healthy hosts are increasing, for which surgical procedures are essential in… read more here.
Keywords: infection; proliferation; vulnificus; signature tagged ... See more keywords