Absolute Protein Quantification by Mass Spectrometry: Not as Simple as Advertised.
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DOI: 10.1021/acs.analchem.7b00858
Abstract: Stable isotopically labeled (SIL) tryptic peptides, cleavable SIL peptides, and a full-length SIL protein were compared for internal calibration (i.e., as internal calibrators) and external calibration (i.e., as internal standards) when quantifying three forms of… read more here.
Keywords: protein; sil; absolute protein; digestion ... See more keywords