P139 HLA antibody identification by Luminex single antigen – Interlaboratory comparison
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.199
Abstract: Aim Single antigen bead (SAB) assays are widely used for histocompatibility testing to detect and measure the strength of HLA antibodies. The aim of this study is to compare the SAB data among labs to… read more here.
Keywords: strength; class; antibody; among labs ... See more keywords
P150 Utilization of two different Luminex single antigen beads assays (SAB) platforms in patients with unusual reactivity patterns
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.210
Abstract: Luminex Single Antigen Beads Assay (SAB) are currently the most widely used means to identify HLA antibodies in sera from sensitized transplant candidates. Although these assays greatly enhance solid organ transplantation practices in terms of… read more here.
Keywords: antigen beads; unusual reactivity; reactivity; luminex single ... See more keywords
P195 Single antigen dilution reveals interference and falsely low MFI for donor specific antibodies
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.255
Abstract: A 48 year old man presented for second kidney transplant with a potential living donor, his brother. His first transplant was in 2000. At evaluation, the patient had HLA single antigen class I and II testing… read more here.
Keywords: dilution; dqa1; serum; donor ... See more keywords
Identification of a recurrent pattern of false-positivity by Luminex HLA MHC class I single antigen bead testing.
Sign Up to like & getrecommendations! Published in 2020 at "Human immunology"
DOI: 10.1016/j.humimm.2019.12.006
Abstract: Previously, a distinct MHC class II Luminex-single antigen bead (SAB) pattern was described and attributed to antibodies targeting denatured antigens. In this study, we describe a distinct MHC class I reactivity pattern observed in 1.8%… read more here.
Keywords: pattern; single antigen; mhc class;
Antibodies to cryptic epitopes on HLA class I and class II heavy chains bound to single antigen beads: Clinically relevant?
Sign Up to like & getrecommendations! Published in 2021 at "Transplant immunology"
DOI: 10.1016/j.trim.2021.101482
Abstract: Cell surface HLA class I consists of trimers, i.e., alpha - heavy chain, beta - 2 - microglobulin, and a peptide, termed closed conformers (CC) on non-activated lymphocytes. HLA class I and class II may… read more here.
Keywords: lifecodes sabs; class; antigen beads; hla class ... See more keywords
CDC (compliment dependent cytotoxicity testing) and Donor Specific Antibody (DSA) Detection by Single Antigen Beads (SAB) Assay for Acute Antibody Mediated Rejection (ABMR) Risk Assessment
Sign Up to like & getrecommendations! Published in 2017 at "Transplantation"
DOI: 10.1097/01.tp.0000520368.38205.54
Abstract: Forty-two patients were evaluated for donor specific anti HLA sensi tion before they recivied ABO incompatible kidney transplantation. E recipient was evaluated by complement dependent cytotoxicity (C crossmatch, flow crossmatch, single antigen bead assay (SAB)… read more here.
Keywords: antibody; dependent cytotoxicity; abmr; antibody mediated ... See more keywords
Conformational Variants of HLA-I Antigens on Luminex Single Antigen Beads.
Sign Up to like & getrecommendations! Published in 2017 at "Transplantation"
DOI: 10.1097/tp.0000000000001607
Abstract: describing the use of monoclonal antibodies for determining the relative proportions of HLA-I conformational variants displayed by Luminex Single Antigen Beads (LSAB). They confirmed previous results showing that (1) LSAB display significant amounts of HLA-I… read more here.
Keywords: tfl 006; antigen beads; luminex single; conformational variants ... See more keywords
Comparison of Two Luminex Single-antigen Bead Flow Cytometry Assays for Detection of Donor-specific Antibodies After Renal Transplantation
Sign Up to like & getrecommendations! Published in 2019 at "Transplantation"
DOI: 10.1097/tp.0000000000002351
Abstract: Background. Defining the clinical relevance of donor-specific HLA antibodies (DSA) detection by Luminex single-antigen (LSA) flow beads assay is critical in monitoring posttransplant outcome. Methods. Sera of kidney transplanted patients were tested by LSA1 and… read more here.
Keywords: test; donor specific; single antigen; detection ... See more keywords
Deciphering the role of the conjugate's phycoerythrin label in complement-mediated interference occurring in HLA single antigen Luminex bead assays.
Sign Up to like & getrecommendations! Published in 2022 at "HLA"
DOI: 10.1111/tan.14628
Abstract: Complement-mediated interference is a well described phenomenon in single antigen bead (SAB) Luminex assay that leads to falsely low or negative results for anti-HLA antibody (Ab). In a context of high amount of Ab, the… read more here.
Keywords: role; mediated interference; interference; single antigen ... See more keywords
Assessing the allogenic realness of the Cw1/12/15 pattern occurring in the LABScreen single antigen assay.
Sign Up to like & getrecommendations! Published in 2023 at "HLA"
DOI: 10.1111/tan.15025
Abstract: Several technical limitations of Luminex single antigen (LSA) assays have been described so far. This study focused on a reactivity pattern observed in many sera that cannot be explained by eplets described in the Epitope… read more here.
Keywords: labscreen single; single antigen; cw1 pattern; pattern ... See more keywords
A modeling approach for mean fluorescence intensity value harmonization and cutoff prediction for luminex single antigen bead assays of two different vendors.
Sign Up to like & getrecommendations! Published in 2023 at "HLA"
DOI: 10.1111/tan.15082
Abstract: Luminex single antigen bead (SAB) kits from One Lambda (OL) and Lifecodes (LC) are widely used for HLA antibody detection but have substantial differences in design and assay protocol resulting in different mean fluorescence intensity… read more here.
Keywords: luminex single; class; single antigen; mfi ... See more keywords