Specific and robust hybridization based on double-stranded nucleic acids with single-base resolution.
Sign Up to like & getrecommendations! Published in 2022 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2022.339568
Abstract: Nucleic acid hybridization plays a critical role in medical diagnostics and nanotechnology, but its selectivity and robustness remain to be improved. Here, focusing on double-stranded nucleic acid-based hybridization, we present a series of related strategies.… read more here.
Keywords: hybridization; double stranded; nucleic acids; stranded nucleic ... See more keywords
Heterotrimeric PCNA increases the activity and fidelity of Dbh, a Y-family translesion DNA polymerase prone to creating single-base deletion mutations.
Sign Up to like & getrecommendations! Published in 2020 at "DNA repair"
DOI: 10.1016/j.dnarep.2020.102967
Abstract: Dbh is a Y-family translesion DNA polymerase from Sulfolobus acidocaldarius, an archaeal species that grows in harsh environmental conditions. Biochemically, Dbh displays a distinctive mutational profile, creating single-base deletion mutations at extraordinarily high frequencies (up… read more here.
Keywords: base deletion; deletion mutations; single base; dna ... See more keywords
Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiological research"
DOI: 10.1016/j.micres.2019.03.007
Abstract: Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that… read more here.
Keywords: aspergillus niger; single base; base; crispr cas9 ... See more keywords
Aligner mediated cleavage of nucleic acids for site-specific detection of single base mismatch.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2019.03.106
Abstract: Single base mismatch can always connect with various gene-related diseases, whose determination has aroused widespread interest. So far, various methods have been developed to determine the common base mismatch. However most of them are complex,… read more here.
Keywords: cleavage; single base; site; base mismatch ... See more keywords
Selective Chemical Labeling and Sequencing of 5-Carboxylcytosine in DNA at Single-Base Resolution.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03201
Abstract: 5-Carboxylcytosine (5caC) plays a vital role in the dynamics of DNA demethylation, sequencing of its sites will help us dig out more biological functions of 5caC. Herein, we present a novel chemical method to efficiently… read more here.
Keywords: base resolution; chemical; single base; dna ... See more keywords
Ultrasensitive and Single-Base Resolution Quantification of 8-Oxo-7,8-dihydroguanine in DNA by Extension and Ligation-Based qPCR.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01679
Abstract: Oxidative DNA damage is tightly linked to the development of multiple age-related diseases. The prominent oxidation product is 8-oxo-7,8-dihydroguanine (OG), which has been proved to be an important epigenetic-like biomarker. Quantification of the locus-specific OG… read more here.
Keywords: base resolution; quantification; dna; oxo dihydroguanine ... See more keywords
Bisulfite-Free and Single-Base Resolution Detection of Epigenetic DNA Modification of 5-Methylcytosine by Methyltransferase-Directed Labeling with APOBEC3A Deamination Sequencing.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03808
Abstract: DNA methylation (5-methylcytosine, 5mC) is the most prevalent epigenetic modification that is predominantly found in CG dinucleotides in mammalian genomes. In-depth investigation of the functions of 5mC heavily relies on the quantitative measurement of 5mC… read more here.
Keywords: base resolution; dna; single base; mlad seq ... See more keywords
Simultaneous Discrimination of Single-Base Mismatch and Full Match Using a Label-Free Single-Molecule Strategy.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01285
Abstract: Identification of single-base mismatches has found wide applications in disease diagnosis, pharmacogenetics, and population genetics. However, there is still a great challenge in the simultaneous discrimination of single-base mismatch and full match. Combined with a… read more here.
Keywords: mismatch full; base; discrimination single; simultaneous discrimination ... See more keywords
An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection
Sign Up to like & getrecommendations! Published in 2018 at "Analytical Methods"
DOI: 10.1039/c8ay00746b
Abstract: Gene point mutations are commonly associated with the development of cancer and other diseases. The rapid and accurate detection of mutation is increasingly needed in personalized therapeutic regimes. In this proof-of-concept study, an isothermal single… read more here.
Keywords: detection; gene point; point; single base ... See more keywords
Aqueous Wittig reaction-mediated fast fluorogenic identification and single-base resolution analysis of 5-formylcytosine in DNA.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/d0cc04950f
Abstract: A highly reactive ylide tBuA was introduced, which could react rapidly with the 5-formyl and 4-amino groups of 5-formylcytosine (5fC) under mild conditions without any co-solvent or catalyst in a manner of Wittig olefination and… read more here.
Keywords: aqueous wittig; base resolution; single base; dna ... See more keywords
A bifunctional chemical signature enabling RNA 4-thiouridine enrichment sequencing with single-base resolution.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d1cc06080e
Abstract: Both sequence enrichment and base resolution are essential for accurate sequencing analysis of low-abundance RNA. Yet they are hindered by the lack of molecular tools. Here we report a bifunctional chemical signature for RNA 4-thiouridine… read more here.
Keywords: base resolution; chemical signature; single base; enrichment ... See more keywords