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   Articles with "single base" as a keyword



High‐throughput detection of RNA modifications at single base resolution

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recommendations! Published in 2024 at "Febs Letters"

DOI: 10.1002/1873-3468.15052

Abstract: RNA is modified by > 170 chemical modifications that affect its structure and function. Accordingly, RNA modifications have been implicated in regulation of gene expression and cellular outcomes in a variety of species spanning the phylogenetic… read more here.

Keywords: high throughput; base resolution; single base; rna ... See more keywords

Canine RNF170 Single Base Deletion in a Naturally Occurring Model for Human Neuroaxonal Dystrophy

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recommendations! Published in 2024 at "Movement Disorders"

DOI: 10.1002/mds.29977

Abstract: Neuroaxonal dystrophy (NAD) is a group of inherited neurodegenerative disorders characterized primarily by the presence of spheroids (swollen axons) throughout the central nervous system. In humans, NAD is heterogeneous, both clinically and genetically. NAD has… read more here.

Keywords: base deletion; neuroaxonal dystrophy; single base; rnf170 single ... See more keywords
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Specific and robust hybridization based on double-stranded nucleic acids with single-base resolution.

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recommendations! Published in 2022 at "Analytica chimica acta"

DOI: 10.1016/j.aca.2022.339568

Abstract: Nucleic acid hybridization plays a critical role in medical diagnostics and nanotechnology, but its selectivity and robustness remain to be improved. Here, focusing on double-stranded nucleic acid-based hybridization, we present a series of related strategies.… read more here.

Keywords: hybridization; double stranded; nucleic acids; stranded nucleic ... See more keywords
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Heterotrimeric PCNA increases the activity and fidelity of Dbh, a Y-family translesion DNA polymerase prone to creating single-base deletion mutations.

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recommendations! Published in 2020 at "DNA repair"

DOI: 10.1016/j.dnarep.2020.102967

Abstract: Dbh is a Y-family translesion DNA polymerase from Sulfolobus acidocaldarius, an archaeal species that grows in harsh environmental conditions. Biochemically, Dbh displays a distinctive mutational profile, creating single-base deletion mutations at extraordinarily high frequencies (up… read more here.

Keywords: base deletion; deletion mutations; single base; dna ... See more keywords

Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion.

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recommendations! Published in 2019 at "Microbiological research"

DOI: 10.1016/j.micres.2019.03.007

Abstract: Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that… read more here.

Keywords: aspergillus niger; single base; base; crispr cas9 ... See more keywords

Aligner mediated cleavage of nucleic acids for site-specific detection of single base mismatch.

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recommendations! Published in 2019 at "Talanta"

DOI: 10.1016/j.talanta.2019.03.106

Abstract: Single base mismatch can always connect with various gene-related diseases, whose determination has aroused widespread interest. So far, various methods have been developed to determine the common base mismatch. However most of them are complex,… read more here.

Keywords: cleavage; single base; site; base mismatch ... See more keywords

Selective Chemical Labeling and Sequencing of 5-Carboxylcytosine in DNA at Single-Base Resolution.

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recommendations! Published in 2020 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.0c03201

Abstract: 5-Carboxylcytosine (5caC) plays a vital role in the dynamics of DNA demethylation, sequencing of its sites will help us dig out more biological functions of 5caC. Herein, we present a novel chemical method to efficiently… read more here.

Keywords: base resolution; chemical; single base; dna ... See more keywords

Ultrasensitive and Single-Base Resolution Quantification of 8-Oxo-7,8-dihydroguanine in DNA by Extension and Ligation-Based qPCR.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c01679

Abstract: Oxidative DNA damage is tightly linked to the development of multiple age-related diseases. The prominent oxidation product is 8-oxo-7,8-dihydroguanine (OG), which has been proved to be an important epigenetic-like biomarker. Quantification of the locus-specific OG… read more here.

Keywords: base resolution; quantification; dna; oxo dihydroguanine ... See more keywords

Bisulfite-Free and Single-Base Resolution Detection of Epigenetic DNA Modification of 5-Methylcytosine by Methyltransferase-Directed Labeling with APOBEC3A Deamination Sequencing.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c03808

Abstract: DNA methylation (5-methylcytosine, 5mC) is the most prevalent epigenetic modification that is predominantly found in CG dinucleotides in mammalian genomes. In-depth investigation of the functions of 5mC heavily relies on the quantitative measurement of 5mC… read more here.

Keywords: base resolution; dna; single base; mlad seq ... See more keywords

Bisulfite-Free and Quantitative Detection of DNA Methylation at Single-Base Resolution by eROS1-seq.

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recommendations! Published in 2024 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.4c05030

Abstract: 5-Methylcytosine (5mC) is the most significant DNA modification present in mammalian genomes. Understanding the roles of 5mC in diverse biological processes requires quantitative detection at single-base resolution. In this study, we engineered the repressor of… read more here.

Keywords: detection; base resolution; quantitative detection; single base ... See more keywords

Simultaneous Discrimination of Single-Base Mismatch and Full Match Using a Label-Free Single-Molecule Strategy.

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recommendations! Published in 2018 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.8b01285

Abstract: Identification of single-base mismatches has found wide applications in disease diagnosis, pharmacogenetics, and population genetics. However, there is still a great challenge in the simultaneous discrimination of single-base mismatch and full match. Combined with a… read more here.

Keywords: mismatch full; base; discrimination single; simultaneous discrimination ... See more keywords