A Laser Driven Microrobot for Thermal Stimulation of Single Cells.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202300904
Abstract: Here, we present the Thermally Activated Cell-Signal Imaging (TACSI) microrobot, capable of photothermal actuation, sensing, and light-driven locomotion. The plasmonic soft microrobot is specifically designed for thermal stimulation of mammalian cells to investigate cell behavior… read more here.
Keywords: laser driven; cell; driven microrobot; stimulation ... See more keywords
Nanomechanical Induction of Autophagy-Related Fluorescence in Single Cells with Atomic Force Microscopy.
Sign Up to like & getrecommendations! Published in 2021 at "Advanced science"
DOI: 10.1002/advs.202102989
Abstract: Mechanistic understanding of how living systems sense, transduce, and respond to mechanical cues has important implications in development, physiology, and therapy. Here, the authors use an integrated atomic force microscope (AFM) and brightfield/epifluorescent microscope platform… read more here.
Keywords: fluorescence; microscopy; atomic force; force ... See more keywords
Quantitative single‐cell optical technologies
Sign Up to like & getrecommendations! Published in 2021 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24463
Abstract: Cells are the fundamental building bricks for all life. Optical technologies have served as the mainstream for single-cell analysis, which may trace back to the 1600s when Robert Hooke reported “cell” in his groundbreaking publication… read more here.
Keywords: single cell; microscopy; single cells; flow cytometry ... See more keywords
Characterization of a novel microfluidic platform for the isolation of rare single cells to enable CTC analysis from head and neck squamous cell carcinoma patients
Sign Up to like & getrecommendations! Published in 2022 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202100133
Abstract: Detailed examination of tumor components is leading‐edge to establish personalized cancer therapy. Accompanying research on cell‐free DNA, the cell count of circulating tumor cells (CTCs) in patient blood is seen as a crucial prognostic factor.… read more here.
Keywords: ctc analysis; cell; isolation; single cells ... See more keywords
Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Small"
DOI: 10.1002/smll.201702955
Abstract: Controlled encapsulation and pairing of single cells within a confined 3D matrix can enable the replication of the highly ordered cellular structure of human tissues. Microgels with independently controlled compartments that can encapsulate cells within… read more here.
Keywords: encapsulation pairing; multicompartment microgels; pairing single; single cells ... See more keywords
Inkjet-Patterned Microdroplets as Individual Microenvironments for Adherent Single Cell Culture.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202107992
Abstract: Adhesion of single cells is the foundation of manifold cellular behaviors and life processes. However, investigating the function of a specific cell is still challenging due to deficiency of adhesion or interference from surrounding cells.… read more here.
Keywords: adherent single; cell; inkjet patterned; single cell ... See more keywords
Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1771-7_17
Abstract: Understanding the genomic landscape of cancer in single cells can be valuable for the characterization of molecular events that drive evolution of tumorigenesis and fostering progress in identifying druggable regimens for patient treatment scenarios. We… read more here.
Keywords: cells using; dna rna; single cells; simultaneous dna ... See more keywords
Lighting-up RNA aptamer transcription synchronization amplification for ultrasensitive and label-free imaging of microRNA in single cells.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.12.040
Abstract: Sensitive imaging of intracellular microRNAs (miRNAs) in cells is of great significance in clinical diagnoses and disease treatments, and it remains a major challenge to achieve this goal. Herein, we report a new in situ… read more here.
Keywords: lighting rna; rna aptamer; transcription synchronization; label free ... See more keywords
Mesoderm specification and diversification: from single cells to emergent tissues.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2019.07.012
Abstract: The three germ layers - mesoderm, endoderm and ectoderm - constituting the cellular blueprint for the tissues and organs that will form during embryonic development, are specified at gastrulation. Cells of mesodermal origin are the… read more here.
Keywords: mesoderm specification; diversification single; single cells; specification diversification ... See more keywords
Design principles of tissue organisation: How single cells coordinate across scales.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2020.07.004
Abstract: Cells act as building blocks of multicellular organisms, forming higher-order structures at different biological scales. Niches, tissues and, ultimately, entire organisms consist of single cells that remain in constant communication. Emergence of developmental patterns and… read more here.
Keywords: cells coordinate; design principles; coordinate across; single cells ... See more keywords
NF-κB Dynamics Discriminate between TNF Doses in Single Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Cell systems"
DOI: 10.1016/j.cels.2017.10.011
Abstract: Although cytokine-dependent dynamics of nuclear factor κB (NF-κB) are known to encode information that regulates cell fate decisions, it is unclear whether single-cell responses are switch-like or encode more information about cytokine dose. Here, we… read more here.
Keywords: single cell; information; discriminate tnf; dynamics discriminate ... See more keywords