A Single Enzyme in Enantiocomplementary Synthesis of β‐Nitroalcohols: Bidirectional Catalysis by Hydroxynitrile Lyase
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202400618
Abstract: A single enzyme, Baliospermum montanum hydroxynitrile lyase (BmHNL), without alteration, enabled bidirectional catalysis in enantiocomplementary synthesis of chiral β‐nitroalcohols. BmHNL catalyzed promiscuous Henry (24 examples) and retro‐Henry reaction (22 examples) provided up to >99 % and… read more here.
Keywords: hydroxynitrile lyase; bidirectional catalysis; synthesis; single enzyme ... See more keywords
Dual Responsive Molecular-Arm Modified Single Enzyme Molecules for Efficient Cellulose Hydrolysis.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular rapid communications"
DOI: 10.1002/marc.202200092
Abstract: Immobilizing cellulase for improving its hydrolysis activity and recyclability is critical for a cost-effective and environment-friendly conversion of cellulosic biomass. However, developing a strategy for achieving a high mass-transfer rate and good separation efficiency between… read more here.
Keywords: cellulase; dual responsive; single enzyme; efficiency ... See more keywords
PCR-RFLP for authentication of different species of processed snappers using mitochondrial D-loop region by single enzyme
Sign Up to like & getrecommendations! Published in 2018 at "Food Control"
DOI: 10.1016/j.foodcont.2018.02.028
Abstract: Abstract A PCR-RFLP protocol was developed for authenticating nine different snapper species by targeting the 515 bp fragment in highly polymorphic mitochondrial D-loop region with a newly designed primer Fish-DL-F/Fish-DL-R, followed by restriction digestion using… read more here.
Keywords: pcr rflp; loop region; single enzyme; mitochondrial loop ... See more keywords
The peroxidase-mimicking function of acetate and its application in single-enzyme-based glucose test paper.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2018.12.064
Abstract: Acetate ion was widely used in pH buffer to control pH environment. Here we firstly found that acetate ion had mimic peroxidase activity. Acetate ions are capable of catalyzing the decomposition of hydrogen peroxide and… read more here.
Keywords: peroxidase; based glucose; enzyme based; single enzyme ... See more keywords
Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-10211-x
Abstract: Reverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread applications… read more here.
Keywords: dna polymerase; polymerase; reverse; reverse transcription ... See more keywords
Sequential single-enzyme oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylicacid by an engineered lanthanide-dependent alcohol dehydrogenase
Sign Up to like & getrecommendations! Published in 2025 at "Green Chemistry"
DOI: 10.1039/d5gc00157a
Abstract: 2,5-furandicarboxylic acid (FDCA) is a potential platform chemical from renewable feedstocks to make various polymers and high-value compounds. Bioconversion of 5-hydroxymethylfurfural (HMF) to FDCA by biocatalysts has the advantages of... read more here.
Keywords: oxidation hydroxymethylfurfural; enzyme oxidation; sequential single; furandicarboxylicacid engineered ... See more keywords