Extending applications of AFM to fluidic AFM in single living cell studies
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.30809
Abstract: In this article, a review of a series of applications of atomic force microscopy (AFM) and fluidic Atomic Force Microscopy (fluidic AFM, hereafter fluidFM) in single‐cell studies is presented. AFM applications involving single‐cell and extracellular… read more here.
Keywords: living cell; cell; afm; microscopy ... See more keywords
Longterm renal allograft survival after sequential liver‐kidney transplantation from a single living donor
Sign Up to like & getrecommendations! Published in 2017 at "Liver Transplantation"
DOI: 10.1002/lt.24676
Abstract: Combined liver‐kidney transplantation (CLKT) is well established as a definitive therapy with the potential to provide complete recovery for certain liver‐kidney diseases, although the results might be contingent on the cause of transplantation. The purposes… read more here.
Keywords: liver kidney; survival; transplantation; single living ... See more keywords
Fluorescent Polymerase Chain Reaction Nanokit for the Detection of DNA Sequence in Single Living Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02470
Abstract: Here, a fluorescent polymerase chain reaction (PCR) nanokit is established to detect the specific DNA sequence in a single living cell. Different from well-developed protocols to load cell-permeable probes into single cell for recognition, the… read more here.
Keywords: single living; detection; dna; dna sequence ... See more keywords
Specially Resolved Single Living Cell Perfusion and Targeted Fluorescence Labeling Based on Nanopipettes.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02537
Abstract: Targeted delivery and labeling of single living cells in heterogeneous cell populations are of great importance to understand the molecular biology and physiological functions of individual cells. However, it remains challenging to perfuse fluorescence markers… read more here.
Keywords: cell; single living; fluorescence labeling; fluorescence ... See more keywords
Probing Queuosine Modifications of Transfer RNA in Single Living Cells via Plasmonic Affinity Sandwich Assay.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02784
Abstract: Queuosine (Q) modification on tRNA plays an essential role in protein synthesis, participating in many tRNA functions such as folding, stability, and decoding. Appropriate analytical tools for the measurement of tRNA Q modifications are essential… read more here.
Keywords: affinity sandwich; affinity; sandwich assay; plasmonic affinity ... See more keywords
Probing Protein 4'-Phosphopantetheinylation in Single Living Cells.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00080
Abstract: 4'-Phosphopantetheinylation (4PPTylation) of proteins, which is derived from the hydrolysis of coenzyme A (CoA), is an essential post-translational modification participating in biosynthetic and metabolic pathways. However, due to the lack of specific recognition ligands as… read more here.
Keywords: phosphopantetheinylation; aldh1l1 single; single living; living cells ... See more keywords
Functionalized Quartz Nanopipette for Intracellular Superoxide Sensing: A Tool for Monitoring Reactive Oxygen Species Levels in Single Living Cell.
Sign Up to like & getrecommendations! Published in 2018 at "ACS sensors"
DOI: 10.1021/acssensors.8b00185
Abstract: Reactive oxygen species (ROS), including superoxide radical anions, are vital components in numerous biological functions, including cell signaling and immune responses. Since ROS react with other biomolecules and oxidize them quickly, it is essential for… read more here.
Keywords: reactive oxygen; oxygen species; single living; superoxide ... See more keywords
O-GlcNAcylation mapping of single living cells by in situ quantitative SERS imaging
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d2sc03881a
Abstract: O-GlcNAcylation is involved in many biological processes including cancerization. Nevertheless, its in situ quantification in single living cells is still a bottleneck. Here we develop a quantitative SERS imaging strategy for mapping the O-GlcNAcylation distribution… read more here.
Keywords: quantitative sers; sers imaging; single living; living cells ... See more keywords