Analysis of Single Nuclei in a Microfluidic Cytometer Towards Metaphase Enrichment
Sign Up to like & getrecommendations! Published in 2025 at "ELECTROPHORESIS"
DOI: 10.1002/elps.8152
Abstract: Identifying analyzable metaphase chromosomes is crucial for karyotyping, a common procedure used by clinicians to diagnose genetic disorders and some forms of cancer. This task is often laborious and time‐consuming, making it essential to develop… read more here.
Keywords: nuclei microfluidic; metaphase enrichment; cytometer towards; analysis single ... See more keywords
Functional Specificity of Astrocyte Subtypes in Alzheimer’s Disease: Decoding Disease Mechanisms Through Network-based Analysis of Integrated Single-Nuclei Multi-Omic Data
Sign Up to like & getrecommendations! Published in 2025 at "Molecular Neurobiology"
DOI: 10.1007/s12035-025-04965-8
Abstract: Alzheimer’s disease (AD) is the most common cause of dementia. Recent studies have revealed incontrovertible roles of astrocytes in the pathology of AD. Considering the conflicting behaviours of astrocytes in AD brain, they have been… read more here.
Keywords: disease; analysis; astrocyte subtypes; single nuclei ... See more keywords
Single nuclei transcriptomics in human and non-human primate striatum in opioid use disorder
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-45165-7
Abstract: In brain, the striatum is a heterogenous region involved in reward and goal-directed behaviors. Striatal dysfunction is linked to psychiatric disorders, including opioid use disorder (OUD). Striatal subregions are divided based on neuroanatomy, each with… read more here.
Keywords: opioid use; use disorder; single nuclei; striatum ... See more keywords
Disentangling glial diversity in peripheral nerves at single-nuclei resolution.
Sign Up to like & getrecommendations! Published in 2022 at "Nature neuroscience"
DOI: 10.1038/s41593-021-01005-1
Abstract: The peripheral nerve contains diverse cell types that support its proper function and maintenance. In this study, we analyzed multiple peripheral nerves using single-nuclei RNA sequencing, which allowed us to circumvent difficulties encountered in analyzing… read more here.
Keywords: diversity; single nuclei; peripheral nerve; cell types ... See more keywords
A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-90070-8
Abstract: Clinical samples are vital for understanding diseases, but their scarcity requires refined research methods. Emerging single-cell technologies offer detailed views of tissue heterogeneity but need sufficient fully characterized tissues. We developed an optimized single-nuclei RNA… read more here.
Keywords: versatile efficient; isolate nuclei; efficient method; single nuclei ... See more keywords
Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2221526120
Abstract: Ruminants have a semi-invasive placenta, which possess highly vascularized placentomes formed by maternal endometrial caruncles and fetal placental cotyledons and required for fetal development to term. The synepitheliochorial placenta of cattle contains at least two… read more here.
Keywords: cell; trophoblast cell; cell types; placenta ... See more keywords
The nesprin-cytoskeleton interface probed directly on single nuclei is a mechanically rich system
Sign Up to like & getrecommendations! Published in 2017 at "Nucleus"
DOI: 10.1080/19491034.2017.1322237
Abstract: ABSTRACT The cytoskeleton provides structure and plays an important role in cellular function such as migration, resisting compression forces, and transport. The cytoskeleton also reacts to physical cues such as fluid shear stress or extracellular… read more here.
Keywords: cytoskeleton; interface probed; nesprin cytoskeleton; cytoskeleton interface ... See more keywords
Single-nuclei sequencing of Moricandia arvensis reveals bundle sheath cell function in the photorespiratory shuttle of C3-C4 intermediate Brassicaceae
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Experimental Botany"
DOI: 10.1093/jxb/eraf245
Abstract: Abstract Spatially confined gene expression determines cell identity and is fundamental to complex plant traits. In the evolutionary transition from C3 to the more efficient C4 photosynthesis, restricting the glycine decarboxylase reaction to bundle sheath… read more here.
Keywords: bundle sheath; sheath; single nuclei; cell ... See more keywords
EPCO-20. RELATING GLIOBLASTOMA HETEROGENEITY TO HUMAN FETAL GLIAL DEVELOPMENT THROUGH HIGH RESOLUTION SINGLE NUCLEI TRANSCRIPTOMICS
Sign Up to like & getrecommendations! Published in 2020 at "Neuro-oncology"
DOI: 10.1093/neuonc/noaa215.299
Abstract: Glioblastoma (GBM) is thought to be driven by a therapy-resistant cancer stem cell population that recapitulates developmental phenotypes. Direct comparisons of GBM to glial states during human fetal development are limited due to paucity of… read more here.
Keywords: resolution; nuclei; gbm; human fetal ... See more keywords
ATRT-28. SINGLE NUCLEI SEQUENCING REVEALS THE DIFFERENT PHENOTYPIC COMPOSITION OF THE ATRT SUBGROUPS
Sign Up to like & getrecommendations! Published in 2020 at "Neuro-Oncology"
DOI: 10.1093/neuonc/noaa222.027
Abstract: Abstract Atypical teratoid/rhabdoid tumors (ATRT) represents a genomically homogeneous disease characterized by loss of SMARCB1 protein in the vast majority of cases. In recent years, it has become clear that these tumors display a high… read more here.
Keywords: atrt; nuclei sequencing; phenotypic composition; single nuclei ... See more keywords
Abstract 6840: Automated preparation of single nuclei from FFPE samples for snRNA-Seq
Sign Up to like & getrecommendations! Published in 2024 at "Cancer Research"
DOI: 10.1158/1538-7445.am2024-6840
Abstract: S2 Genomics developed the Singulator™ 100 and 200 Systems to automate the processing of fresh, frozen, or OCT tissues into single cell or single nuclei suspensions using disposable cartridges, reagents, and customizable protocols. The systems… read more here.
Keywords: snrna seq; preparation; single nuclei; ffpe ... See more keywords