Single‐Nucleus RNA Sequencing and Spatial Transcriptomics Reveal the Immunological Microenvironment of Cervical Squamous Cell Carcinoma
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203040
Abstract: Effective treatment of advanced invasive cervical cancer remains challenging nowadays. Herein, single-nucleus RNA sequencing (snRNA-seq) and SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq) technology are used to investigate the immunological microenvironment of cervical squamous cell carcinoma (CSCC),… read more here.
Keywords: immunological microenvironment; rna sequencing; microenvironment; single nucleus ... See more keywords
Single-nucleus transcriptomic landscape of primate hippocampal aging
Sign Up to like & getrecommendations! Published in 2021 at "Protein & Cell"
DOI: 10.1007/s13238-021-00852-9
Abstract: The hippocampus plays a crucial role in learning and memory, and its progressive deterioration with age is functionally linked to a variety of human neurodegenerative diseases. Yet a systematic profiling of the aging effects on… read more here.
Keywords: single nucleus; nucleus transcriptomic; primate hippocampal; hippocampal aging ... See more keywords
Single-nucleus Hi-C of mammalian oocytes and zygotes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.032
Abstract: The 3D folding of the genome is linked to essential nuclear processes including gene expression, DNA repair, and replication. Chromatin conformation capture assays such as Hi-C are providing unprecedented insights into higher-order chromatin structure. Bulk… read more here.
Keywords: single nucleus; nucleus mammalian; chromatin; oocytes zygotes ... See more keywords
Massively Parallel Single Nucleus Transcriptional Profiling Defines Spinal Cord Neurons and Their Activity during Behavior
Sign Up to like & getrecommendations! Published in 2018 at "Cell reports"
DOI: 10.1016/j.celrep.2018.02.003
Abstract: SUMMARY To understand the cellular basis of behavior, it is necessary to know the cell types that exist in the nervous system and their contributions to function. Spinal networks are essential for sensory processing and… read more here.
Keywords: single nucleus; parallel single; massively parallel; activity ... See more keywords
PBX/Knotted 1 homeobox-2 (PKNOX2) is a novel regulator of myocardial fibrosis
Sign Up to like & getrecommendations! Published in 2024 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-024-01804-5
Abstract: Much effort has been made to uncover the cellular heterogeneities of human hearts by single-nucleus RNA sequencing. However, the cardiac transcriptional regulation networks have not been systematically described because of the limitations in detecting transcription… read more here.
Keywords: novel regulator; fibrosis; pknox2; single nucleus ... See more keywords
Single-nucleus and spatial transcriptomics identify brain landscape of gene regulatory networks associated with behavioral maturation in honeybees
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-58614-8
Abstract: Animal behavior is linked to the gene regulatory network (GRN) coordinating gene expression in the brain. Eusocial honeybees, with their natural behavioral plasticity, provide an excellent model for exploring the connection between brain activity and… read more here.
Keywords: brain; behavioral maturation; gene; single nucleus ... See more keywords
Single-nucleus and spatial signatures of the brainstem in normal brain and mild traumatic brain injury in male mice
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-59856-2
Abstract: The mammalian brainstem is particularly vulnerable to mild traumatic brain injury (mTBI), which is associated with prolonged autonomic dysfunction and coma. The spatial cellular connections within the brainstem or the mechanisms underlying its response to… read more here.
Keywords: traumatic brain; brain; brain injury; injury ... See more keywords
Single-nucleus transcriptomics reveal the morphogenesis and artemisinin biosynthesis in Artemisia annua glandular trichomes
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-63770-y
Abstract: Artemisinin, the key antimalarial drug, is synthesized in Artemisia annua glandular secretory trichomes (GSTs), yet their development and artemisinin’s precise cellular origins are unclear. Utilizing single-nucleus RNA sequencing and spatial transcriptomics, we construct a high-resolution… read more here.
Keywords: artemisinin biosynthesis; annua glandular; artemisia annua; biosynthesis ... See more keywords
Single-nucleus and spatial transcriptomic profiling of human temporal cortex and white matter reveals key associations with AD pathology
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-65350-6
Abstract: Alzheimer’s disease, the leading cause of dementia in the elderly, is a neurodegenerative disorder that has been studied to uncover therapeutic pathways through its molecular and cellular hallmarks. Here, we present a comprehensive investigation of… read more here.
Keywords: temporal cortex; white matter; nucleus spatial; pathology ... See more keywords
Cumulus provides cloud-based data analysis for large-scale single-cell and single-nucleus RNA-seq
Sign Up to like & getrecommendations! Published in 2020 at "Nature methods"
DOI: 10.1038/s41592-020-0905-x
Abstract: Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for computational pipelines… read more here.
Keywords: single cell; large scale; single nucleus; cell single ... See more keywords
An integrated single-nucleus and spatial transcriptomics atlas reveals the molecular landscape of the human hippocampus
Sign Up to like & getrecommendations! Published in 2025 at "Nature Neuroscience"
DOI: 10.1038/s41593-025-02022-0
Abstract: Cell types in the hippocampus with unique morphology, physiology and connectivity serve specialized functions associated with cognition and mood. These cell types are spatially organized, necessitating molecular profiling strategies that retain cytoarchitectural organization. Here we… read more here.
Keywords: snrna seq; organization; hippocampus; human hippocampus ... See more keywords