Single‐Nucleus RNA Sequencing and Spatial Transcriptomics Reveal the Immunological Microenvironment of Cervical Squamous Cell Carcinoma
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203040
Abstract: Effective treatment of advanced invasive cervical cancer remains challenging nowadays. Herein, single-nucleus RNA sequencing (snRNA-seq) and SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq) technology are used to investigate the immunological microenvironment of cervical squamous cell carcinoma (CSCC),… read more here.
Keywords: immunological microenvironment; rna sequencing; microenvironment; single nucleus ... See more keywords
Single-nucleus transcriptomic landscape of primate hippocampal aging
Sign Up to like & getrecommendations! Published in 2021 at "Protein & Cell"
DOI: 10.1007/s13238-021-00852-9
Abstract: The hippocampus plays a crucial role in learning and memory, and its progressive deterioration with age is functionally linked to a variety of human neurodegenerative diseases. Yet a systematic profiling of the aging effects on… read more here.
Keywords: single nucleus; nucleus transcriptomic; primate hippocampal; hippocampal aging ... See more keywords
Single-nucleus Hi-C of mammalian oocytes and zygotes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.032
Abstract: The 3D folding of the genome is linked to essential nuclear processes including gene expression, DNA repair, and replication. Chromatin conformation capture assays such as Hi-C are providing unprecedented insights into higher-order chromatin structure. Bulk… read more here.
Keywords: single nucleus; nucleus mammalian; chromatin; oocytes zygotes ... See more keywords
Massively Parallel Single Nucleus Transcriptional Profiling Defines Spinal Cord Neurons and Their Activity during Behavior
Sign Up to like & getrecommendations! Published in 2018 at "Cell reports"
DOI: 10.1016/j.celrep.2018.02.003
Abstract: SUMMARY To understand the cellular basis of behavior, it is necessary to know the cell types that exist in the nervous system and their contributions to function. Spinal networks are essential for sensory processing and… read more here.
Keywords: single nucleus; parallel single; massively parallel; activity ... See more keywords
Cumulus provides cloud-based data analysis for large-scale single-cell and single-nucleus RNA-seq
Sign Up to like & getrecommendations! Published in 2020 at "Nature methods"
DOI: 10.1038/s41592-020-0905-x
Abstract: Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for computational pipelines… read more here.
Keywords: single cell; large scale; single nucleus; cell single ... See more keywords
Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00514-4
Abstract: Single-cell and single-nucleus sequencing techniques are a burgeoning field with various biological, biomedical and clinical applications. Numerous high- and low-throughput methods have been developed for sequencing the RNA and DNA content of single cells. However,… read more here.
Keywords: single cell; single nucleus; protocol; extraction nuclei ... See more keywords
Enhancing droplet-based single-nucleus RNA-seq resolution using the semi-supervised machine learning classifier DIEM
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-67513-5
Abstract: Single-nucleus RNA sequencing (snRNA-seq) measures gene expression in individual nuclei instead of cells, allowing for unbiased cell type characterization in solid tissues. We observe that snRNA-seq is commonly subject to contamination by high amounts of… read more here.
Keywords: seq; single nucleus; diem; rna ... See more keywords
Quantification and statistical modeling of droplet-based single-nucleus RNA-sequencing data.
Sign Up to like & getrecommendations! Published in 2023 at "Biostatistics"
DOI: 10.1093/biostatistics/kxad010
Abstract: In complex tissues containing cells that are difficult to dissociate, single-nucleus RNA-sequencing (snRNA-seq) has become the preferred experimental technology over single-cell RNA-sequencing (scRNA-seq) to measure gene expression. To accurately model these data in downstream analyses,… read more here.
Keywords: snrna seq; rna sequencing; seq; single nucleus ... See more keywords
A single-nucleus transcriptomic atlas of the dog hippocampus reveals the potential relationship between specific cell types and domestication
Sign Up to like & getrecommendations! Published in 2022 at "National Science Review"
DOI: 10.1093/nsr/nwac147
Abstract: Abstract The process of domestication has led to dramatic differences in behavioral traits between domestic dogs and gray wolves. Whole-genome research found that a class of putative positively selected genes were related to various aspects… read more here.
Keywords: atlas dog; domestication; cell; transcriptomic atlas ... See more keywords
Single-nucleus transcriptomics reveals a gatekeeper role for FOXP1 in primate cardiac aging
Sign Up to like & getrecommendations! Published in 2022 at "Protein & Cell"
DOI: 10.1093/procel/pwac038
Abstract: Abstract Aging poses a major risk factor for cardiovascular diseases, the leading cause of death in the aged population. However, the cell type-specific changes underlying cardiac aging are far from being clear. Here, we performed… read more here.
Keywords: cell; single nucleus; primate cardiac; cardiac aging ... See more keywords
A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasis.
Sign Up to like & getrecommendations! Published in 2022 at "Protein & cell"
DOI: 10.1093/procel/pwac057
Abstract: The testis is pivotal for male reproduction, and its progressive functional decline in aging is associated with infertility. However, the regulatory mechanism underlying primate testicular aging remains largely elusive. Here, we resolve the aging-related cellular… read more here.
Keywords: cell; single nucleus; primate testicular; nucleus transcriptomic ... See more keywords