Transmission of poly(dT60) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3315
Abstract: The objective of this study was to examine membrane filtration of a single stranded DNA (ssDNA) with 60 thymine nucleotides, and to elucidate the variables controlling its transmission across track‐etched porous membranes. Dead end filtration… read more here.
Keywords: single stranded; ssdna; size; ionic strength ... See more keywords
G‐quadruplex‐enhanced circular single‐stranded DNA (G4‐CSSD) adsorption of miRNA to inhibit colon cancer progression
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Medicine"
DOI: 10.1002/cam4.5721
Abstract: Chromosomal heterogeneity leads to the abnormal expression and mutation of tumor‐specific genes. Drugs targeting oncogenes have been extensively developed. However, given the random mutation of tumor suppressor genes, the development of its targeted drugs is… read more here.
Keywords: single stranded; quadruplex enhanced; stranded dna; enhanced circular ... See more keywords
Principles of Amino‐Acid‐Nucleotide Interactions Revealed by Binding Affinities between Homogeneous Oligopeptides and Single‐Stranded DNA Molecules
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200048
Abstract: We have determined the binding strengths between nucleotides of adenine, thymine, guanine and cytosine in homogeneous single stranded DNAs and homo‐octapeptides consisting of 20 common amino acids. We use a bead‐based fluorescence assay for these… read more here.
Keywords: acid nucleotide; single stranded; dna; principles amino ... See more keywords
Human immunodeficiency virus Type‐1 single‐stranded RNA activates the NLRP3 inflammasome and impairs autophagic clearance of damaged mitochondria in human microglia
Sign Up to like & getrecommendations! Published in 2019 at "Glia"
DOI: 10.1002/glia.23568
Abstract: Despite the availability of antiretroviral therapy (ART) that fully suppresses human immunodeficiency virus type‐1 (HIV), markers of inflammation and minor neurocognitive impairment are frequently identified in HIV‐infected persons. Increasing data support that low‐level replication defective… read more here.
Keywords: single stranded; human immunodeficiency; virus type; immunodeficiency virus ... See more keywords
An Efficient Bead‐captured Denaturation Method for Preparing Long Single‐stranded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Journal of The Chinese Chemical Society"
DOI: 10.1002/jccs.201700142
Abstract: Previous methods to prepare single-stranded DNA (ssDNA) substrates are limited to short DNA lengths and inefficient. We have developed an efficient and rapid method to prepare long ssDNA substrates (up to 4000 nt) based on the… read more here.
Keywords: single stranded; dna; ssdna; bead captured ... See more keywords
The intrinsically disordered linker of E. coli SSB is critical for the release from single‐stranded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3115
Abstract: The Escherichia coli single stranded DNA binding protein (SSB) is crucial for DNA replication, recombination and repair. Within each process, it has two seemingly disparate roles: it stabilizes single‐stranded DNA (ssDNA) intermediates generated during DNA… read more here.
Keywords: single stranded; dna; ssb; stranded dna ... See more keywords
Two different kinds of interaction modes of deaminase APOBEC3A with single‐stranded DNA in solution detected by nuclear magnetic resonance
Sign Up to like & getrecommendations! Published in 2021 at "Protein Science"
DOI: 10.1002/pro.4242
Abstract: APOBEC3A (A3A) deaminates deoxycytidine in target motif TC in a single‐stranded DNA (we termed it as TC DNA), which mortally mutates viral pathogens and immunoglobulins, and leads to the diversification and lethality of cancers. The… read more here.
Keywords: dna; stranded dna; two different; solution ... See more keywords
Single-Stranded DNA-Binding Proteins in the Archaea.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_2
Abstract: Single-stranded (ss) DNA-binding proteins are found in all three domains of life where they play vital roles in nearly all aspects of DNA metabolism by binding to and stabilizing exposed ssDNA and acting as platforms… read more here.
Keywords: single stranded; ssdna binding; binding; stranded dna ... See more keywords
Measuring the Complex Effects of the Single-Stranded DNA-Binding Protein gp2.5 on Primer Synthesis and Extension by the Bacteriophage T7 Replisome.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_21
Abstract: The single-stranded DNA-binding protein gp2.5 of bacteriophage T7 plays myriad functions in the replication of phage genomes. In addition to interacting with ssDNA, gp2.5 binds to the T7 DNA polymerase and primase/helicase proteins, regulating their… read more here.
Keywords: single stranded; gp2 primer; protein gp2; stranded dna ... See more keywords
Structural Characterization of a Single-Stranded DNA-Binding Protein: A Case Study of the ORF6 Protein from Bacteriophage Enc34.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_23
Abstract: In the quest to understand how single-stranded DNA-binding proteins function and evolve at a molecular level, determination of their high-resolution three-dimensional structure using methods such as X-ray crystallography is indispensable. Here we present a collection… read more here.
Keywords: single stranded; biology; stranded dna; binding protein ... See more keywords
Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7339-2_3
Abstract: Identification of sequences preferred by individual RNA-binding proteins (RBPs) has been accelerated by recent advances in the quantitative analysis of protein-RNA interactions on a massive scale, and such experiments have even revealed hidden sequence specificity… read more here.
Keywords: single stranded; vitro loading; stranded rnas; deep sequencing ... See more keywords