SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.04.032
Abstract: In vitro single-vesicle fusion assays are important tools to analyze the details of SNARE-mediated fusion processes. In this study, we employed planar pore-spanning membranes (PSMs) prepared on porous silicon substrates with large pore diameters of 5… read more here.
Keywords: single vesicle; snare mediated; vesicle fusion; vesicle ... See more keywords
Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis
Sign Up to like & getrecommendations! Published in 2025 at "ACS Nano"
DOI: 10.1021/acsnano.5c00706
Abstract: Extracellular vesicles (EVs) play a crucial role in intercellular communication, signaling pathways, and disease pathogenesis by transporting biomolecules such as DNA, RNA, proteins, and lipids derived from their cells of origin, and they have demonstrated… read more here.
Keywords: extracellular vesicles; analysis; bulk; clinical diagnostics ... See more keywords
Vitamin C Drives Reentrant Actin Phase Transition: Biphasic Exocytosis Regulation Revealed by Single-Vesicle Electrochemistry.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.4c02710
Abstract: Unveiling molecular mechanisms that dominate protein phase dynamics has been a pressing need for deciphering the intricate intracellular modulation machinery. While ions and biomacromolecules have been widely recognized for modulating protein phase separations, effects of… read more here.
Keywords: protein phase; electrochemistry; single vesicle; drives reentrant ... See more keywords
Single-Vesicle Electrochemistry in Fresh Brain Slices Enables In Situ Quantification of Vesicular Monoamine.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.4c06572
Abstract: The quantitative analysis of vesicular neurotransmitters in neurons in situ is paramount for investigating neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease (PD). Unfortunately, a direct approach for monitoring neurotransmitter chemistry in single vesicles… read more here.
Keywords: methodology; brain; electrochemistry; single vesicle ... See more keywords
Single-vesicle Tracking of α‑Synuclein Oligomers Reveals Pore Formation by a Three-Stage Model
Sign Up to like & getrecommendations! Published in 2025 at "ACS Nano"
DOI: 10.1101/2025.04.10.648112
Abstract: Neurodegenerative disorders like Parkinson’s disease (PD) pose significant health challenges. A major hallmark is the aggregation of α-synuclein into toxic oligomers (αSO) and fibrils. While many efforts focus on slowing disease progression, the molecular origins… read more here.
Keywords: pore formation; stage model; three stage; single vesicle ... See more keywords
Determination of membrane protein orientation upon liposomal reconstitution down to the single vesicle level
Sign Up to like & getrecommendations! Published in 2023 at "Biological Chemistry"
DOI: 10.1515/hsz-2022-0325
Abstract: Abstract Reconstitution of membrane proteins into liposomal membranes represents a key technique in enabling functional analysis under well-defined conditions. In this review, we provide a brief introduction to selected methods that have been developed to… read more here.
Keywords: reconstitution; protein orientation; membrane protein; membrane ... See more keywords
Single Vesicle Surface Protein Profiling and Machine Learning-Based Dual Image Analysis for Breast Cancer Detection
Sign Up to like & getrecommendations! Published in 2024 at "Nanomaterials"
DOI: 10.3390/nano14211739
Abstract: Single-vesicle molecular profiling of cancer-associated extracellular vesicles (EVs) is increasingly being recognized as a powerful tool for cancer detection and monitoring. Mask and target dual imaging is a facile method to quantify the fraction of… read more here.
Keywords: cancer; breast cancer; analysis; machine learning ... See more keywords