SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.04.032
Abstract: In vitro single-vesicle fusion assays are important tools to analyze the details of SNARE-mediated fusion processes. In this study, we employed planar pore-spanning membranes (PSMs) prepared on porous silicon substrates with large pore diameters of 5… read more here.
Keywords: single vesicle; snare mediated; vesicle fusion; vesicle ... See more keywords
Determination of membrane protein orientation upon liposomal reconstitution down to the single vesicle level
Sign Up to like & getrecommendations! Published in 2023 at "Biological Chemistry"
DOI: 10.1515/hsz-2022-0325
Abstract: Abstract Reconstitution of membrane proteins into liposomal membranes represents a key technique in enabling functional analysis under well-defined conditions. In this review, we provide a brief introduction to selected methods that have been developed to… read more here.
Keywords: reconstitution; protein orientation; membrane protein; membrane ... See more keywords