The Sister-Chromatid Exchange Assay in Human Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0644-5_26
Abstract: The semiconservative nature of DNA replication allows the differential labeling of sister chromatids that is the fundamental requirement to perform the sister-chromatid exchange (SCE) assay. SCE assay is a powerful technique to visually detect the… read more here.
Keywords: sister chromatid; dna; sister; chromatid exchange ... See more keywords
Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6545-8_5
Abstract: In sexually reproducing organisms, the formation of healthy gametes (sperm and eggs) requires the proper establishment and release of meiotic sister chromatid cohesion (SCC). SCC tethers replicated sisters from their formation in premeiotic S phase… read more here.
Keywords: cohesion; chromatid cohesion; sister chromatid; meiotic sister ... See more keywords
Revealing Sister Chromatid Interactions with the loxP/Cre Recombination Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7098-8_3
Abstract: Sister chromatid cohesion is a transient state during replication in bacteria. It has been recently demonstrated that the extent of contact between cohesive sisters during the cell cycle is dependent on topoisomerase IV activity, suggesting… read more here.
Keywords: sister; revealing sister; sister chromatid; recombination assay ... See more keywords
Sister Chromatid Exchange as a Genotoxic Stress Marker.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9432-8_7
Abstract: Sister chromatid exchange (SCE) is the phenomenon of partial DNA exchange during DNA replication. SCE detection has been developed through eliciting DNA's semiconservative replicative nature. Thymidine analogues such as 5'-bromodeoxyuridine (BrdU) and ethynyldeoxyuridine (EdU) are incorporated into… read more here.
Keywords: sister chromatid; dna; chromatid exchange; exchange ... See more keywords
Mutagenic and genotoxic effects of Anilofos with micronucleus, chromosome aberrations, sister chromatid exchanges and Ames test
Sign Up to like & getrecommendations! Published in 2017 at "Cytotechnology"
DOI: 10.1007/s10616-017-0099-y
Abstract: We aimed to evaluate the mutagenic effect of Anilofos, organophosphate pesticide, by using Ames/Salmonella/microsome test. Its cytotoxic and genotoxic effects were also determined by chromosome aberration (CA), sister chromatid exchange (SCE) and micronucleus (MN) test… read more here.
Keywords: sister chromatid; genotoxic effects; 100 200; genotoxic ... See more keywords
Maternal age-dependent APC/C-mediated decrease in securin causes premature sister chromatid separation in meiosis II
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms15346
Abstract: Sister chromatid attachment during meiosis II (MII) is maintained by securin-mediated inhibition of separase. In maternal ageing, oocytes show increased inter-sister kinetochore distance and premature sister chromatid separation (PSCS), suggesting aberrant separase activity. Here, we… read more here.
Keywords: sister chromatid; securin; meiosis; sister ... See more keywords
DNA double-strand breaks in telophase lead to coalescence between segregated sister chromatid loci
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10742-8
Abstract: DNA double strand breaks (DSBs) pose a high risk for genome integrity. Cells repair DSBs through homologous recombination (HR) when a sister chromatid is available. HR is upregulated by the cycling dependent kinase (CDK) despite… read more here.
Keywords: sister chromatid; dna double; chromatid; strand breaks ... See more keywords
Cytidine deaminase deficiency impairs sister chromatid disjunction by decreasing PARP-1 activity
Sign Up to like & getrecommendations! Published in 2017 at "Cell Cycle"
DOI: 10.1080/15384101.2017.1317413
Abstract: ABSTRACT Bloom Syndrome (BS) is a rare genetic disease characterized by high levels of chromosomal instability and an increase in cancer risk. Cytidine deaminase (CDA) expression is downregulated in BS cells, leading to an excess… read more here.
Keywords: chromatid disjunction; sister chromatid; parp activity; cytidine deaminase ... See more keywords
Proper sister chromatid disjunction requires CDA and PARP-1
Sign Up to like & getrecommendations! Published in 2017 at "Cell Cycle"
DOI: 10.1080/15384101.2017.1326767
Abstract: The accurate replication of the human genome in S-phase and the faithful segregation of sister chromatids in mitosis are fundamental events required for the maintenance of chromosome stability. Cells that are copying their DNA in… read more here.
Keywords: sister chromatid; cda; dna; sister ... See more keywords
Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e16-12-0853
Abstract: HPV-transforming protein E6 weakens both SAC activity and sister chromatid cohesion. These processes largely function normally and result in chromosome segregation errors only when mitosis is delayed by the presence of one to four unaligned,… read more here.
Keywords: chromatid cohesion; sister chromatid; hpv;
Preprint Highlight: Cohesin mediates DNA loop extrusion and sister chromatid cohesion by distinct mechanisms
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.p23-03-0010
Abstract: Cohesins organize chromatin within the nucleus by promoting loop extrusion via cis contacts on chromatin and sister chromatid cohesion via trans contacts between chromatids. Whether cohesins employ a distinct mechanism for these two functions is… read more here.
Keywords: extrusion; sister chromatid; chromatid cohesion; loop extrusion ... See more keywords