Loop engineering reveals the importance of active-site-decorating loops and gating residue in substrate affinity modulation of arginine deiminase (an anti-tumor enzyme).
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DOI: 10.1016/j.bbrc.2018.03.134
Abstract: Protein engineering of enzyme loop regions is an effective strategy to improve enzymatic properties. Previous studies that aimed to boost the activity of PpADI (an arginine deiminase from Pseudomonas plecoglossicida) under physiological conditions yielded several… read more here.
Keywords: substrate affinity; active site; arginine deiminase; site ... See more keywords