Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6472-7_22
Abstract: Constructing protein-coding genes with desired mutations is a basic step for protein engineering. Herein, we describe a multiple site-directed and saturation mutagenesis method, termed MUPAC. This method has been used to introduce multiple site-directed mutations… read more here.
Keywords: saturation mutagenesis; multiple site; directed saturation; site directed ... See more keywords
Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6472-7_24
Abstract: Site-directed mutagenesis is a commonly used molecular biology technique to manipulate biological sequences, and is especially useful for studying sequence determinants of enzyme function or designing proteins with improved activity. We describe a strategy using… read more here.
Keywords: using gibson; biology; site directed; directed mutagenesis ... See more keywords
High-Throughput Site-Directed Mutagenesis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_13
Abstract: Protein engineering has an array of uses: whether you are studying a disease mutation, removing undesirable sequences, adding stabilizing mutations for structural purposes, or simply dissecting protein function. Protein engineering is almost exclusively performed using… read more here.
Keywords: throughput; site directed; directed mutagenesis; high throughput ... See more keywords
A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method
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DOI: 10.1007/s00253-020-10477-3
Abstract: Abstract A PCR-independent in vitro site-directed mutagenesis method was established. Cas12a from Francisella novicida (FnCas12a) linearizes the plasmid with single digestion. T5 exonuclease removes the target nucleotide. A short single- or double-stranded mutagenic oligonucleotide introduces… read more here.
Keywords: mutagenesis; directed mutagenesis; mutagenesis method; site ... See more keywords
Increase of Bacillus badius Phenylalanine dehydrogenase specificity towards phenylalanine substrate by site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2017.10.009
Abstract: Phenylalanine dehydrogenase (PheDH) is a key enzyme in medical diagnostic for determining the amount of phenylalanine to detect phenylketonuria (PKU) disease. However, determination of phenylalanine can be usually disturbed in presence of tyrosine in blood… read more here.
Keywords: directed mutagenesis; phenylalanine dehydrogenase; phenylalanine; site directed ... See more keywords
EPR of site-directed spin-labeled proteins: A powerful tool to study structural flexibility.
Sign Up to like & getrecommendations! Published in 2020 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2020.108323
Abstract: Electron Paramagnetic Resonance is a spectroscopic technique which, in combination with site-directed spin-labeling, provides structural and dynamic information about proteins in conditions similar to those of their physiological environment. The information is sequence-resolved as it… read more here.
Keywords: flexibility; epr site; site; site directed ... See more keywords
Increased activity of β-glucuronidase variants produced by site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2018 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2017.09.005
Abstract: β-glucuronidase (BGus) is an essential glycosyl hydrolase which has been widely used in biological and biomedical applications. In this paper, we report the construction and screening of nineteen Escherichia coli BGus (EBGus) mutants using site-directed… read more here.
Keywords: directed mutagenesis; site; glucuronidase; site directed ... See more keywords
Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2021 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2021.109931
Abstract: Glucose isomerase (GI) is a key enzyme in the preparation of high fructose corn syrup (HFCS). In this study, a mutant TEGI-M-L38 M/V137 L (TEGI-M2) of glucose isomerase (TEGI-M) originated from Thermoanaerobacter ethanalicus CCSD1 was… read more here.
Keywords: glucose isomerase; tegi; directed mutagenesis; site directed ... See more keywords
Improvement of catalytic performance of endoglucanase CgEndo from Colletotrichum graminicola by site-directed mutagenesis.
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DOI: 10.1016/j.enzmictec.2021.109963
Abstract: In order to improve the catalytic efficiency of cellulase for more effective utilization of lignocellulose, a novel endoglucanase (CgEndo) from Colletotrichum graminicola was expressed by Pichia pastoris X33 and modified by site-directed mutagenesis. Two mutants,… read more here.
Keywords: endoglucanase cgendo; directed mutagenesis; endoglucanase; colletotrichum graminicola ... See more keywords
Improved catalytic properties of a serine hydroxymethyl transferase from Idiomarina loihiensis by site directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.05.003
Abstract: A novel glyA gene was screened from a marine bacterium, Idiomarina loihiensis encoding a thermo-stable serine hydroxymethyl transferase (SHMT; 418 AA; 45.4 kDa). The activities of wild type (WT) and mutants were analyzed against d-phenylserine using… read more here.
Keywords: serine hydroxymethyl; idiomarina loihiensis; site; hydroxymethyl transferase ... See more keywords
Improving the enzyme property of ornithine transcarbamylase from Lactobacillus brevis through site-directed mutation
Sign Up to like & getrecommendations! Published in 2020 at "Lwt - Food Science and Technology"
DOI: 10.1016/j.lwt.2020.109953
Abstract: Abstract Ornithine transcarbamylase (OTC) is an enzyme that participates in the degradation of arginine by catalyzing the formation of ornithine and carbamoyl phosphate from citrulline, which is a major precursor of ethyl carbamate (EC), a… read more here.
Keywords: directed mutation; ornithine; site directed; ornithine transcarbamylase ... See more keywords