Site‐Specific Polyplex on CCR7 Down‐Regulation and T Cell Elevation for Lymphatic Metastasis Blocking on Breast Cancer
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202201166
Abstract: Tumor metastasis contributes to high cancer mortality. Tumor cells in lymph nodes (LNs) are difficult to eliminate but underlie uncontrollable systemic metastasis. The CC chemokine receptor 7 (CCR7) is overexpressed in tumor cells and interacts… read more here.
Keywords: metastasis; ccr7; tumor cells; site specific ... See more keywords
A Proresolving Peptide Nanotherapy for Site‐Specific Treatment of Inflammatory Bowel Disease by Regulating Proinflammatory Microenvironment and Gut Microbiota
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201900610
Abstract: Abstract The incidence and prevalence of inflammatory bowel disease (IBD) increases steadily worldwide. There is an urgent need for effective and safe IBD therapies. Accelerated resolution of inflammation is a new strategy for the management… read more here.
Keywords: bowel disease; inflammatory bowel; site specific; gut microbiota ... See more keywords
Engineering active lysostaphin variants that incorporate noncanonical amino acids and characterizing the effects of site‐specific PEGylation
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28360
Abstract: We describe a facile strategy to identify sites for the incorporation of noncanonical amino acids into lysostaphin—an enzyme that degrades the cell wall of Staphylococcus aureus—while retaining stapholytic activity. We used this strategy to generate… read more here.
Keywords: amino acids; lysostaphin variants; site specific; site ... See more keywords
Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28852
Abstract: The development of bone implants through bioinspired immobilization of growth factors remains a key issue in the generation of biological interfaces, especially in enhancing osteodifferentiation ability. In this study, we developed a strategy for surface… read more here.
Keywords: site specific; growth; growth factor; bmp2 ... See more keywords
A Novel, Site‐Specific N‐Linked Glycosylation Model Provides Mechanistic Insights Into the Process‐Condition Dependent Distinct Fab and Fc Glycosylation of an IgG1 Monoclonal Antibody Produced by CHO VRC01 Cells
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28916
Abstract: The CHO VRC01 cell line produces an anti‐HIV IgG1 monoclonal antibody containing N‐linked glycans on both the Fab (variable) and Fc (constant) regions. Site‐specific glycan analysis was used to measure the complex effects of cell… read more here.
Keywords: specific linked; model; site specific; linked glycosylation ... See more keywords
Generation of a host cell line containing a MAR‐rich landing pad for site‐specific integration and expression of transgenes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3254
Abstract: In recent years, targeted gene integration (TI) has been introduced as a strategy for the generation of recombinant mammalian cell lines for the production of biotherapeutics. Besides reducing the immense heterogeneity within a pool of… read more here.
Keywords: site specific; cell; host cell; specific integration ... See more keywords
A genetic off‐target event in a site‐specific integration cell line expressing monoclonal antibody has no impact on commercial suitability
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3320
Abstract: Site‐specific integration (SSI) cell line systems are gaining popularity for biotherapeutic development and production. Despite the proven advantages for these expression hosts, the SSI system is still susceptible to rare off‐target events and potential vector… read more here.
Keywords: cell; cell line; integration; site specific ... See more keywords
Site‐Specific Incorporation of Chemical Fluorescence on Live Enterovirus‐71 Virion by Using an Organometallic Palladium Reagent To Monitor Virus Entry
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800051
Abstract: Imaging live virus to monitor the viral entry process is essential to understand virus–host interactions during pathogen infection. However, methods for efficient labeling of live viruses, in particular labeling non‐enveloped viruses and tracing virus entry… read more here.
Keywords: organometallic palladium; site specific; virus entry; entry ... See more keywords
Site‐Specific Protein Labeling with Fluorophores as a Tool To Monitor Protein Turnover
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.201900651
Abstract: Proteins that terminally fail to acquire their native structure are detected and degraded by cellular quality control systems. Insights into cellular protein quality control are key to a better understanding of how cells establish and… read more here.
Keywords: protein; protein turnover; site specific; monitor protein ... See more keywords
Site‐Specific Selenocysteine Incorporation into Proteins by Genetic Engineering
Sign Up to like & getrecommendations! Published in 2021 at "ChemBioChem"
DOI: 10.1002/cbic.202100124
Abstract: Selenocysteine (Sec), a rare naturally proteinogenic amino acid, is the major form of essential trace element selenium in living organisms. Selenoproteins, with one or several Sec residues, are found in all three domains of life.… read more here.
Keywords: selenocysteine incorporation; site specific; specific selenocysteine; incorporation ... See more keywords
Site‐Specific Anchoring a Luminescent Tag in a Protein with Non‐Emissive Iridium(III) Complex
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202300798
Abstract: Site‐specific modification of proteins with synthetic fluorescent tag effectively improves the resolution of imaging, and such a labeling method with negligible three‐dimensional structural perturbations and minimal impact on the biological functions of proteins is of… read more here.
Keywords: emissive iridium; iii; protein; iridium iii ... See more keywords