Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201900023
Abstract: The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N‐linked glycans. We aimed to readily attach defined N‐glycans to EPO through copper‐catalyzed azide alkyne cycloaddition. EPO variants… read more here.
Keywords: conjugated complex; site specifically; synthesis erythropoietins; site ... See more keywords
Generation and Characterization of Site‐Specifically Mono‐Ubiquitylated p53
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202100659
Abstract: The tumor suppressor p53 is regulated by various posttranslational modifications including different types of ubiquitylation, which exert distinct effects on p53. While modification by ubiquitin chains targets p53 for degradation, attachment of single ubiquitin moieties… read more here.
Keywords: mono ubiquitylated; site specifically; specifically mono; generation characterization ... See more keywords
Labeling Proteins at Site-Specifically Incorporated 5-Hydroxytryptophan Residues Using a Chemoselective Rapid Azo-Coupling Reaction.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9654-4_16
Abstract: Chemoselective protein labeling is a valuable tool in the arsenal of modern chemical biology. The unnatural amino acid mutagenesis technology provides a powerful way to site-specifically introduce nonnatural chemical functionalities into recombinant proteins, which can… read more here.
Keywords: rapid azo; chemoselective rapid; site specifically; biology ... See more keywords
Quantitative PET imaging of PD-L1 expression in xenograft and syngeneic tumour models using a site-specifically labelled PD-L1 antibody
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Nuclear Medicine and Molecular Imaging"
DOI: 10.1007/s00259-019-04646-4
Abstract: Purpose Despite remarkable clinical responses and prolonged survival across several cancers, not all patients benefit from PD-1/PD-L1 immune checkpoint blockade. Accordingly, assessment of tumour PD-L1 expression by immunohistochemistry (IHC) is increasingly applied to guide patient… read more here.
Keywords: tumour models; pet imaging; site specifically; dfo 6e11 ... See more keywords
Interactome of Site-Specifically Acetylated Linker Histone H1.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.1c00396
Abstract: Linker histone H1 plays a key role in chromatin organization and maintenance, yet our knowledge of the regulation of H1 functions by post-translational modifications is rather limited. In this study, we report on the generation… read more here.
Keywords: site specifically; interactome site; site; linker histone ... See more keywords
Site-Specifically-Labeled Antibodies for Super-Resolution Microscopy Reveal In Situ Linkage Errors
Sign Up to like & getrecommendations! Published in 2021 at "ACS Nano"
DOI: 10.1021/acsnano.1c03677
Abstract: The precise spatial localization of proteins in situ by super-resolution microscopy (SRM) demands their targeted labeling. Positioning reporter molecules as close as possible to the target remains a challenge in primary cells or tissues from… read more here.
Keywords: microscopy; super resolution; site specifically; resolution microscopy ... See more keywords
Bioactive Site-Specifically Modified Proteins for 4D Patterning of Gel Biomaterials
Sign Up to like & getrecommendations! Published in 2019 at "Nature materials"
DOI: 10.1038/s41563-019-0367-7
Abstract: Protein-modified biomaterials can be used to modulate cellular function in three dimensions. However, as the dynamic heterogeneous control over complex cell physiology continues to be sought, strategies that permit a reversible and user-defined tethering of… read more here.
Keywords: specifically modified; bioactive site; site specifically; modified proteins ... See more keywords
Semisynthesis of site-specifically succinylated histone reveals that succinylation regulates nucleosome unwrapping rate and DNA accessibility
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa663
Abstract: Abstract Posttranslational modifications (PTMs) of histones represent a crucial regulatory mechanism of nucleosome and chromatin dynamics in various of DNA-based cellular processes, such as replication, transcription and DNA damage repair. Lysine succinylation (Ksucc) is a… read more here.
Keywords: succinylation; dna; site specifically; semisynthesis site ... See more keywords