Methods for In Situ Protein Visualization in Dental Mineralized Tissues.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9012-2_17
Abstract: Immunohistochemistry (IHC) is a technique based on the specificity of antibody-antigen principle used commonly to detect antigens in tissue sections. The immune labeling can be performed in paraffin sections, cryostat sections, and ultrathin sections and… read more here.
Keywords: protein visualization; mineralized tissues; tissues methods; methods situ ... See more keywords
CRISPR Lights up In Situ Protein Evolution.
Sign Up to like & getrecommendations! Published in 2020 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2020.05.005
Abstract: In this issue of Cell Chemical Biology, Erdogan et al. (2020) describe a new CRISPR/Cas9-based strategy for performing directed evolution of mammalian proteins in situ. Using this technique to select functional mRuby3 variants within lysosomes, they… read more here.
Keywords: crispr; evolution; lights situ; biology ... See more keywords
Advanced fluorescence imaging of in situ protein aggregation.
Sign Up to like & getrecommendations! Published in 2020 at "Physical biology"
DOI: 10.1088/1478-3975/ab694e
Abstract: The aggregation of intrinsically disordered proteins is a hallmark of neurodegenerative diseases, such as Alzheimer's, Parkinson's and Huntington's disease. Although we currently have a good molecular level understanding on how protein aggregation occurs in vitro,… read more here.
Keywords: aggregation; advanced fluorescence; protein aggregation; biology ... See more keywords