Simultaneous determination of the styrene unit content and assessment of molecular weight of triblock copolymers in adhesives by a size exclusion chromatography method.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of separation science"
DOI: 10.1002/jssc.201700306
Abstract: The content of styrene units in nonhydrogenated and hydrogenated styrene-butadiene-styrene and styrene-isoprene-styrene triblock copolymers significantly influences product performance. A size exclusion chromatography method was developed to determine the average styrene content of triblock copolymers blended… read more here.
Keywords: butadiene styrene; styrene; triblock copolymers; spectroscopy ... See more keywords
Comparison of the separation of proteins of wide-ranging molecular weight via trilobal polypropylene capillary-channeled polymer fiber, commercial superficiously porous, and commercial size exclusion columns.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100891
Abstract: Reversed phase and size-exclusion chromatography methods are commonly used for protein separations, though based on distinctly different principles. Reversed phase methods yield hydrophobicity-based (loosely-termed) separation of proteins on porous supports, but tend to be limited… read more here.
Keywords: separation; separation proteins; size exclusion; fiber ... See more keywords
Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Separation Science"
DOI: 10.1002/jssc.202200075
Abstract: The present study describes the possibilities offered by an innovative bioinert size exclusion chromatography column for size variant characterization of complex monoclonal antibody products. This size exclusion chromatography column includes a novel column hardware surface.… read more here.
Keywords: monoclonal antibody; size; size exclusion; exclusion chromatography ... See more keywords
Microfluidic Size Exclusion Chromatography (μSEC) for Extracellular Vesicles and Plasma Protein Separation.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202104470
Abstract: Extracellular vesicles (EVs) are recognized as next generation diagnostic biomarkers due to their disease-specific biomolecular cargoes and importance in cell-cell communications. A major bottleneck in EV sample preparation is the inefficient and laborious isolation of… read more here.
Keywords: extracellular vesicles; chromatography sec; sec; size exclusion ... See more keywords
Well‐Paired‐Seq: A Size‐Exclusion and Locally Quasi‐Static Hydrodynamic Microwell Chip for Single‐Cell RNA‐Seq
Sign Up to like & getrecommendations! Published in 2022 at "Small Methods"
DOI: 10.1002/smtd.202200341
Abstract: Single‐cell RNA sequencing (scRNA‐seq) is a powerful technology for revealing the heterogeneity of cellular states. However, existing scRNA‐seq platforms that utilize bead‐based technologies suffer from a large number of empty microreactors and a low cell/bead… read more here.
Keywords: well paired; cell; paired seq; seq ... See more keywords
High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_17
Abstract: Structural biology has revealed predicting heterologous expression levels, homogeneity, and stability of a protein from its primary structure are exceedingly difficult. Membrane proteins, in particular, present numerous challenges that make obtaining milligram quantities of quality… read more here.
Keywords: membrane proteins; characterization membrane; exclusion chromatography; biology ... See more keywords
Advances on Size Exclusion Chromatography and Applications on the Analysis of Protein Biopharmaceuticals and Protein Aggregates: A Mini Review
Sign Up to like & getrecommendations! Published in 2017 at "Chromatographia"
DOI: 10.1007/s10337-017-3380-5
Abstract: Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characterization of the protein-based products is a complicated and challenging task necessary to guarantee their efficacy and safety. A major concern in manufacturing… read more here.
Keywords: protein; protein aggregates; protein biopharmaceuticals; exclusion chromatography ... See more keywords
Quantification and varietal variation of low molecular weight glutenin subunits (LMW-GS) using size exclusion chromatography
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-017-2995-8
Abstract: Crude glutenin of four commercial wheat varieties viz. C 306, HI 977, HW 2004 and PBW 550 of diverse origin and breadmaking quality were fractionated by size-exclusion chromatography into three fractions of decreasing molecular weights.… read more here.
Keywords: exclusion chromatography; size exclusion; quantification varietal;
Size Exclusion Chromatography-Ion Mobility-Mass Spectrometry Coupling: a Step Toward Structural Biology
Sign Up to like & getrecommendations! Published in 2017 at "Journal of The American Society for Mass Spectrometry"
DOI: 10.1007/s13361-017-1810-0
Abstract: AbstractNoncovalent interactions are essential for the structural organization of biomacromolecules in cells. For this reason, the study of the biophysical, dynamic, and architectural interactions among biomacromolecules is essential. Since mass spectrometry requires compatible solutions while… read more here.
Keywords: mobility; biology; size exclusion; mass spectrometry ... See more keywords
Monitoring WWTP performance using size-exclusion chromatography with simultaneous UV and fluorescence detection to track recalcitrant wastewater fractions.
Sign Up to like & getrecommendations! Published in 2019 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2018.09.099
Abstract: A trial monitoring of a typical full-scale municipal WWTP in Central Finland was aimed to explore applicability of high performance liquid chromatography - size exclusion chromatography (HPSEC) with simultaneous UV and fluorescence detection as a… read more here.
Keywords: fluorescence; exclusion chromatography; simultaneous fluorescence; wastewater ... See more keywords
Investigation of cellulose nitrate and cellulose acetate plastics in museum collections using ion chromatography and size exclusion chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cultural Heritage"
DOI: 10.1016/j.culher.2018.05.011
Abstract: Abstract One of the goals of the Preservation of Plastics Project at the Getty Conservation Institute is to investigate preventative and conservation strategies for objects made of cellulose ester plastics. To achieve this goal, several… read more here.
Keywords: chromatography; ion chromatography; cellulose nitrate; size exclusion ... See more keywords