A Non‐Centrifugation Method to Concentrate and Purify Extracellular Vesicles Using Superabsorbent Polymer Followed by Size Exclusion Chromatography
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.70037
Abstract: ABSTRACT Extracellular vesicles (EVs) can be isolated and purified from cell cultures and biofluids using different methodologies. Here, we explored a novel EV isolation approach by combining superabsorbent polymers (SAP) in a dialysis membrane with… read more here.
Keywords: extracellular vesicles; exclusion chromatography; size exclusion;
Simultaneous determination of the styrene unit content and assessment of molecular weight of triblock copolymers in adhesives by a size exclusion chromatography method.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of separation science"
DOI: 10.1002/jssc.201700306
Abstract: The content of styrene units in nonhydrogenated and hydrogenated styrene-butadiene-styrene and styrene-isoprene-styrene triblock copolymers significantly influences product performance. A size exclusion chromatography method was developed to determine the average styrene content of triblock copolymers blended… read more here.
Keywords: butadiene styrene; styrene; triblock copolymers; spectroscopy ... See more keywords
Comparison of the separation of proteins of wide-ranging molecular weight via trilobal polypropylene capillary-channeled polymer fiber, commercial superficiously porous, and commercial size exclusion columns.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100891
Abstract: Reversed phase and size-exclusion chromatography methods are commonly used for protein separations, though based on distinctly different principles. Reversed phase methods yield hydrophobicity-based (loosely-termed) separation of proteins on porous supports, but tend to be limited… read more here.
Keywords: separation; separation proteins; size exclusion; fiber ... See more keywords
Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Separation Science"
DOI: 10.1002/jssc.202200075
Abstract: The present study describes the possibilities offered by an innovative bioinert size exclusion chromatography column for size variant characterization of complex monoclonal antibody products. This size exclusion chromatography column includes a novel column hardware surface.… read more here.
Keywords: monoclonal antibody; size; size exclusion; exclusion chromatography ... See more keywords
Microfluidic Size Exclusion Chromatography (μSEC) for Extracellular Vesicles and Plasma Protein Separation.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202104470
Abstract: Extracellular vesicles (EVs) are recognized as next generation diagnostic biomarkers due to their disease-specific biomolecular cargoes and importance in cell-cell communications. A major bottleneck in EV sample preparation is the inefficient and laborious isolation of… read more here.
Keywords: extracellular vesicles; chromatography sec; sec; size exclusion ... See more keywords
Well‐Paired‐Seq: A Size‐Exclusion and Locally Quasi‐Static Hydrodynamic Microwell Chip for Single‐Cell RNA‐Seq
Sign Up to like & getrecommendations! Published in 2022 at "Small Methods"
DOI: 10.1002/smtd.202200341
Abstract: Single‐cell RNA sequencing (scRNA‐seq) is a powerful technology for revealing the heterogeneity of cellular states. However, existing scRNA‐seq platforms that utilize bead‐based technologies suffer from a large number of empty microreactors and a low cell/bead… read more here.
Keywords: well paired; cell; paired seq; seq ... See more keywords
Quantifying the effects of size exclusion on colloidal particle transport in porous media at a pore scale and column scale
Sign Up to like & getrecommendations! Published in 2024 at "Vadose Zone Journal"
DOI: 10.1002/vzj2.20388
Abstract: Colloid‐facilitated transport has been widely acknowledged as a significant contributor to surface water and groundwater pollution. Compared to water‐dissolved solutes, colloids are subject to a size exclusion effect (SEE) due to their inherent size when… read more here.
Keywords: size; transport porous; porous media; size exclusion ... See more keywords
High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_17
Abstract: Structural biology has revealed predicting heterologous expression levels, homogeneity, and stability of a protein from its primary structure are exceedingly difficult. Membrane proteins, in particular, present numerous challenges that make obtaining milligram quantities of quality… read more here.
Keywords: membrane proteins; characterization membrane; exclusion chromatography; biology ... See more keywords
Accounting for shear scission in the size-exclusion chromatography of debranched starch
Sign Up to like & getrecommendations! Published in 2025 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-025-05917-w
Abstract: The chain-length distribution (CLD) of starch influences many functional properties of starch-containing substances, and also contains information about starch biosynthetic processes. The commonest method for measuring this CLD is to debranch the starch enzymatically, and… read more here.
Keywords: shear scission; scission; exclusion chromatography; starch ... See more keywords
Advances on Size Exclusion Chromatography and Applications on the Analysis of Protein Biopharmaceuticals and Protein Aggregates: A Mini Review
Sign Up to like & getrecommendations! Published in 2017 at "Chromatographia"
DOI: 10.1007/s10337-017-3380-5
Abstract: Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characterization of the protein-based products is a complicated and challenging task necessary to guarantee their efficacy and safety. A major concern in manufacturing… read more here.
Keywords: protein; protein aggregates; protein biopharmaceuticals; exclusion chromatography ... See more keywords
Quantification and varietal variation of low molecular weight glutenin subunits (LMW-GS) using size exclusion chromatography
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-017-2995-8
Abstract: Crude glutenin of four commercial wheat varieties viz. C 306, HI 977, HW 2004 and PBW 550 of diverse origin and breadmaking quality were fractionated by size-exclusion chromatography into three fractions of decreasing molecular weights.… read more here.
Keywords: exclusion chromatography; size exclusion; quantification varietal;