Feasibility and limitations of cultured skin fibroblasts for germline genetic testing in hematologic disorders.
Sign Up to like & getrecommendations! Published in 2022 at "Human mutation"
DOI: 10.1002/humu.24374
Abstract: To avoid acquired variants found in the blood, cultured skin fibroblasts are a recommended DNA source for germline genetic testing in patients with hematologic disorders, but data are lacking regarding practicality and limitations. We conducted… read more here.
Keywords: genetic testing; germline genetic; skin fibroblasts; cultured skin ... See more keywords
Role of XIST/miR‐29a/LIN28A pathway in denatured dermis and human skin fibroblasts (HSFs) after thermal injury
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.26307
Abstract: Denatured dermis is a part of the dermis in deep burn wound and has the ability to restore normal morphology and function. In our previous study, we revealed that miR‐29a downregulation in denatured dermis may… read more here.
Keywords: mir 29a; denatured dermis; 29a lin28a; human skin ... See more keywords
Proteomic Profiling of Skin Fibroblasts as a Model of Schizophrenia.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-52479-5_29
Abstract: Since many aspects of schizophrenia are also manifested at the peripheral level in proliferating cell types, this chapter describes the analysis of skin fibroblasts biopsied from living patients. The method focuses on cell culture and… read more here.
Keywords: fibroblasts model; skin; proteomic profiling; model schizophrenia ... See more keywords
Exogenous proline stimulates type I collagen and HIF-1α expression and the process is attenuated by glutamine in human skin fibroblasts
Sign Up to like & getrecommendations! Published in 2017 at "Molecular and Cellular Biochemistry"
DOI: 10.1007/s11010-017-3069-y
Abstract: Abundance of proline (Pro) in collagen molecule led us to investigate whether Pro supply affects collagen biosynthesis in human skin fibroblasts. Treatment of the cells with milimolar concentrations (5 and 10 mM) of Pro for 24… read more here.
Keywords: collagen; medium; human skin; expression ... See more keywords
Attenuation of PRRX2 and HEY2 enables efficient conversion of adult human skin fibroblasts to neurons.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.06.089
Abstract: The direct conversion of accessible cells such as human fibroblasts to inaccessible cells, particularly neurons, opens up many opportunities for using the human model system to study diseases and discover therapies. Previous studies have indicated… read more here.
Keywords: hey2; adult human; human skin; conversion ... See more keywords
Decreased amount of vimentin N-terminal truncated proteolytic products in parkin-mutant skin fibroblasts.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.10.154
Abstract: Vimentin, a member of cytoskeleton intermediate filaments proteins, plays a critical role in cell structure and dynamics. The present proteomic study reveals reduced amount of six different lengths, N-terminal truncated proteolytic products of vimentin, in… read more here.
Keywords: amount; truncated proteolytic; terminal truncated; proteolytic products ... See more keywords
Pro-inflammatory cytokines suppress HYBID (hyaluronan (HA) -binding protein involved in HA depolymerization/KIAA1199/CEMIP) -mediated HA metabolism in human skin fibroblasts.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.12.082
Abstract: In the skin, the metabolism of hyaluronan (HA) is highly regulated. Aging leads to chronic low-grade inflammation, which is characterized by elevated levels of pro-inflammatory cytokines; however, the relationship between inflammation and HA metabolism is… read more here.
Keywords: metabolism; human skin; inflammatory cytokines; pro inflammatory ... See more keywords
Impairment of mitochondrial oxidative phosphorylation in skin fibroblasts of SALS and FALS patients is rescued by in vitro treatment with ROS scavengers
Sign Up to like & getrecommendations! Published in 2021 at "Experimental Neurology"
DOI: 10.1016/j.expneurol.2021.113620
Abstract: Amyotrophic lateral sclerosis (ALS) is a devastating, rapidly progressive, neurodegenerative disorder affecting upper and lower motor neurons. Approximately 10% of patients suffer from familial ALS (FALS) with mutations in different ubiquitously expressed genes including SOD1,… read more here.
Keywords: impairment mitochondrial; sals fals; mitochondrial oxidative; oxidative phosphorylation ... See more keywords
Proteomic analysis of rutin effect on human skin fibroblasts exposed to UVA or UVB irradiation
Sign Up to like & getrecommendations! Published in 2017 at "Free Radical Biology and Medicine"
DOI: 10.1016/j.freeradbiomed.2017.04.188
Abstract: Rutin, due to its polyphenolic structure reveals antioxidant properties and may be used as a cytoprotective compound against UV-induced effects on skin cells. Therefore, the aim of this study was to examine the rutin effects… read more here.
Keywords: uvb irradiation; analysis; human skin; rutin ... See more keywords
Proteins involved in the antioxidant and inflammatory response in rutin-treated human skin fibroblasts exposed to UVA or UVB irradiation.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of dermatological science"
DOI: 10.1016/j.jdermsci.2018.02.002
Abstract: BACKGROUND Rutin, due to its polyphenolic structure, has antioxidant properties and can be used as a cytoprotective compound against UV-induced effects on skin cells. OBJECTIVE The aim of this study was to examine the effect… read more here.
Keywords: proteins involved; human skin; inflammatory response; involved antioxidant ... See more keywords
Notch3 protein expression in skin fibroblasts from CADASIL patients
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the Neurological Sciences"
DOI: 10.1016/j.jns.2018.04.027
Abstract: AIM CADASIL is an inherited cerebrovascular disease caused by mutations in the NOTCH3 gene. Notch signaling is involved in a broad spectrum of function, from the cell proliferation to apoptosis. Thus far, because the molecular… read more here.
Keywords: protein expression; cadasil patients; notch3 protein; expression ... See more keywords