Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1835-6_14
Abstract: Argonaute (AGO) proteins associate with small RNAs (sRNAs) to form an RNA-induced silencing complex (RISC). The ribonuclease (slicer) activity of AGOs is required for the sRNA-complementary target cleavage, which is important for RISC-mediated RNA silencing, especially… read more here.
Keywords: slicer; purification; slicer activity; activity ... See more keywords
Crystal structure of Drosophila Piwi
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-14687-1
Abstract: PIWI-clade Argonaute proteins associate with PIWI-interacting RNAs (piRNAs), and silence transposons in animal gonads. Here, we report the crystal structure of the Drosophila PIWI-clade Argonaute Piwi in complex with endogenous piRNAs, at 2.9 Å resolution. A… read more here.
Keywords: crystal structure; structure drosophila; drosophila piwi; piwi ... See more keywords