Methods of Ovarian Tissue Cryopreservation: Is Vitrification Superior to Slow Freezing?—Ovarian Tissue Freezing Methods
Sign Up to like & getrecommendations! Published in 2021 at "Reproductive Sciences"
DOI: 10.1007/s43032-021-00591-6
Abstract: After cancer treatment, female survivors often develop ovarian insufficiency or failure. Oocyte and embryo freezing are well-established fertility preservation options, but cannot be applied in pre-pubescent girls, in women with hormone-sensitive malignancies, or when gonadotoxic… read more here.
Keywords: slow freezing; ovarian tissue; tissue; cryopreservation ... See more keywords
Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.
Sign Up to like & getrecommendations! Published in 2019 at "Acta histochemica"
DOI: 10.1016/j.acthis.2019.03.008
Abstract: This work aimed to evaluate the effect of two distinct cryopreservation procedures - conventional slow-freezing and vitrification, on survivability and mesenchymal marker expression stability of rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs). Cells at passage… read more here.
Keywords: freezing vitrification; expression; slow freezing; rabbit amniotic ... See more keywords
Slow freezing versus vitrification technique for human ovarian tissue cryopreservation: An evaluation of histological changes, WNT signaling pathway and apoptotic genes expression.
Sign Up to like & getrecommendations! Published in 2017 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2017.09.007
Abstract: This study compared slow freezing and vitrification of ovarian tissue by evaluation of histological changes, WNT signaling pathway and apoptotic genes expression. Ovarian tissue was obtained from women aging 27-38 years old. Ovarian cortex from… read more here.
Keywords: wnt signaling; ovarian tissue; slow freezing; expression ... See more keywords
Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.01.002
Abstract: The aim of our investigations was to compare the effectiveness of two methods for cryopreservation of sheep ovarian tissue, slow freezing and vitrification. The quality of cryopreserved tissues was evaluated after 5 days of thawing… read more here.
Keywords: ovarian tissue; slow freezing; freezing vitrification; sheep ovarian ... See more keywords
Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis.
Sign Up to like & getrecommendations! Published in 2021 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2021.06.007
Abstract: Objectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3,473) survival of Bos taurus embryos. The literature was systematically… read more here.
Keywords: slow freezing; survival bos; embryonic survival; post ... See more keywords
Fine visualization of biological cells using X-ray micro-CT with the slow freezing contrast improved method
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-09070-3
Abstract: X-ray computed tomography (CT) is widely used in various fields for the non-destructive three-dimensional (3D) observation of internal structures within objects. However, biological cells are primarily composed of light elements such as oxygen and carbon,… read more here.
Keywords: slow freezing; contrast; biological cells; freezing contrast ... See more keywords
P-325 Comparing slow-freezing and vitrification endometrial biopsy cryopreservation protocols for tissue cryodamage and functional organoid formation
Sign Up to like & getrecommendations! Published in 2024 at "Human Reproduction"
DOI: 10.1093/humrep/deae108.689
Abstract: Are slow-freezing and vitrification suitable methods for cryopreserving endometrial biopsies intended for downstream research applications and organoid formation? Both slow-frozen and vitrified endometrial biopsies resulted in successful organoid formation thus can be considered similar to… read more here.
Keywords: endometrial biopsies; vitrification; slow freezing; freezing vitrification ... See more keywords
Slow Freezing vs. Vitrification of Testicular Tissue in Nontraditional Animal Models.
Sign Up to like & getrecommendations! Published in 2025 at "Biopreservation and biobanking"
DOI: 10.1177/19475535251391026
Abstract: Introduction: Optimal cryopreservation of testicular tissue is essential for species research and conservation, enabling long-term storage of genetic resources through vitrification or slow freezing. Comparing responses from different taxonomic groups to these techniques is crucial… read more here.
Keywords: testicular tissue; vitrification; cryopreservation; slow freezing ... See more keywords
DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.1114695
Abstract: Introduction and objective Cryopreservation of testicular tissues offers new possibilities to protect endangered species, genetically valuable individuals or even the fertility potential of prepubertal individuals who have died unexpectedly. However, the use of this technique… read more here.
Keywords: dna integrity; dna; vitrification; slow freezing ... See more keywords
Freezing Does Not Alter Sperm Telomere Length despite Increasing DNA Oxidation and Fragmentation
Sign Up to like & getrecommendations! Published in 2023 at "Genes"
DOI: 10.3390/genes14051039
Abstract: Correlations were reported between sperm telomere length (STL) and male fertility, sperm DNA fragmentation, and oxidation. Sperm freezing is widely used for assisted reproductive techniques, fertility preservation, and sperm donation. However, its impact on STL… read more here.
Keywords: oxidation; dna; fragmentation; stl ... See more keywords