The Resting Oxidized State of Small Laccase Analyzed with Paramagnetic NMR Spectroscopy
Sign Up to like & getrecommendations! Published in 2021 at "Chemphyschem"
DOI: 10.1002/cphc.202100063
Abstract: Abstract The enzyme laccase catalyzes the reduction of dioxygen to water at the trinuclear copper center (TNC). The TNC comprises a type‐3 (T3) and a type‐2 (T2) copper site. The paramagnetic NMR spectrum of the… read more here.
Keywords: state; paramagnetic nmr; small laccase; resting oxidized ... See more keywords
Enhancing the expression of recombinant small laccase in Pichia pastoris by a double promoter system and application in antibiotics degradation.
Sign Up to like & getrecommendations! Published in 2021 at "Folia microbiologica"
DOI: 10.1007/s12223-021-00894-w
Abstract: Low-expression levels remain a challenge in the quest to use the small laccase (rSLAC) as a viable catalyst. In this study, a recombinant Pichia pastoris strain (rSLAC-GAP-AOX) producing rSLAC under both AOX and GAP promoters… read more here.
Keywords: promoter system; laccase; rslac; small laccase ... See more keywords
Chemical Exchange at the Trinuclear Copper Center of Small Laccase from Streptomyces coelicolor
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2020.05.022
Abstract: The trinuclear copper center (TNC) of laccase reduces oxygen to water with very little overpotential. The arrangement of the coppers and ligands in the TNC is known to be from many crystal structures, yet information… read more here.
Keywords: laccase streptomyces; copper center; laccase; trinuclear copper ... See more keywords
Substitution of the Methionine Axial Ligand of the T1 Copper for the Fungal-like Phenylalanine Ligand (M298F) Causes Local Structural Perturbations that Lead to Thermal Instability and Reduced Catalytic Efficiency of the Small Laccase from Streptomyces coelicolor A3(2)
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c06668
Abstract: Many industrial processes operate at elevated temperatures or within broad pH and salinity ranges. However, the utilization of enzymes to carry out biocatalysis in such processes is often impractical or even impossible. Laccases (EC 1.10.3.2),… read more here.
Keywords: local structural; small laccase; ligand; streptomyces coelicolor ... See more keywords