N-terminal transmembrane-helix epitope tag for x-ray crystallography and electron microscopy of small membrane proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.166909
Abstract: Structural studies of membrane proteins, especially small membrane proteins, are associated with well-known experimental challenges. Complexation with monoclonal antibody fragments is a common strategy to augment such proteins; however, generating antibody fragments that specifically bind… read more here.
Keywords: small membrane; membrane proteins; microscopy; electron microscopy ... See more keywords
The Protein Fusion Strategy Facilitates the Structure Determination of Small Membrane Proteins by Cryo-EM.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00319
Abstract: Despite the resolution revolution of cryo-EM, structures of small membrane proteins ( read more here.
Keywords: fusion; membrane proteins; structure determination; protein fusion ... See more keywords