Assessment of bacterial profiles in aged, home-made Sichuan paocai brine with varying titratable acidity by PacBio SMRT sequencing technology
Sign Up to like & getrecommendations! Published in 2017 at "Food Control"
DOI: 10.1016/j.foodcont.2017.02.006
Abstract: Abstract Sichuan paocai, a traditional Chinese fermented vegetable, is rife with lactic acid bacteria (LAB). However, the precise bacterial profiles of home-made Sichuan paocai brine (HSPB) remain unclear. In this study, the bacterial compositions of… read more here.
Keywords: sichuan paocai; home made; smrt sequencing; acidity ... See more keywords
Single-molecule real-time (SMRT) sequencing facilitates Tachypleus tridentatus genome annotation.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.01.029
Abstract: Tachypleus tridentatus is a keystone species in marine ecosystems. Its hemolymph also provides the limulus amebocyte lysate (LAL) for detection of bacterial endotoxin in human medical service. Here we combined SMRT sequencing and Illumina RNA-seq… read more here.
Keywords: tachypleus tridentatus; smrt sequencing; tridentatus genome; annotation ... See more keywords
Detecting a long insertion variant in SAMD12 by SMRT sequencing: implications of long-read whole-genome sequencing for repeat expansion diseases
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Human Genetics"
DOI: 10.1038/s10038-018-0551-7
Abstract: Long-read sequencing technology is now capable of reading single-molecule DNA with an average read length of more than 10 kb, fully enabling the coverage of large structural variations (SVs). This advantage may pave the way for… read more here.
Keywords: whole genome; long read; smrt sequencing; read whole ... See more keywords
Hb Q-Thailand heterozygosity unlinked with the (-α4.2/) α+-thalassemia deletion allele identified by long-read SMRT sequencing: hematological and molecular analyses.
Sign Up to like & getrecommendations! Published in 2023 at "Hematology"
DOI: 10.1080/16078454.2023.2184118
Abstract: OBJECTIVE In the present study, two unrelated cases of Hb Q-Thailand heterozygosity unlinked with the (-α4.2/) α+-thalassemia deletion allele were identified by long-read single molecule real-time (SMRT) sequencing in southern China. The aim of this… read more here.
Keywords: thalassemia; read smrt; smrt sequencing; deletion allele ... See more keywords
A Sequel to Sanger: amplicon sequencing that scales
Sign Up to like & getrecommendations! Published in 2018 at "BMC Genomics"
DOI: 10.1186/s12864-018-4611-3
Abstract: BackgroundAlthough high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single… read more here.
Keywords: sanger amplicon; sequel sanger; smrt sequencing; amplicon ... See more keywords
A Chinese SCA36 pedigree analysis of NOP56 expansion region based on long-read sequencing
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2023.1110307
Abstract: Introduction: Spinocerebellar ataxias 36 (SCA36) is the neurodegenerative disease caused by the GGCCTG Hexanucleotide repeat expansions in NOP56, which is too long to sequence using short-read sequencing. Single molecule real time (SMRT) sequencing can sequence… read more here.
Keywords: smrt sequencing; read sequencing; expansion; long read ... See more keywords
SMRT Sequencing Revealed Mitogenome Characteristics and Mitogenome-Wide DNA Modification Pattern in Ophiocordyceps sinensis
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2017.01422
Abstract: Single molecule, real-time (SMRT) sequencing was used to characterize mitochondrial (mt) genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt genome of O. sinensis, with a… read more here.
Keywords: sinensis; ophiocordyceps sinensis; dna modification; modification pattern ... See more keywords
Using PacBio SMRT Sequencing Technology and Metabolomics to Explore the Microbiota-Metabolome Interaction Related to Silage Fermentation of Woody Plant
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.857431
Abstract: Silage fermentation is a dynamic process involving the succession of microbial communities and changes in metabolites. Fresh branched and leaves of paper mulberry were used to prepared silage. Crop by-products including corn bran, rice bran,… read more here.
Keywords: silage fermentation; sequencing technology; silage; acid ... See more keywords
The Versatility of SMRT Sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Genes"
DOI: 10.3390/genes10010024
Abstract: The adoption of single molecule real-time (SMRT) sequencing [...]. read more here.
Keywords: smrt sequencing; versatility smrt;
Illumina RNA and SMRT Sequencing Reveals the Mechanism of Uptake and Transformation of Selenium Nanoparticles in Soybean Seedlings
Sign Up to like & getrecommendations! Published in 2023 at "Plants"
DOI: 10.3390/plants12040789
Abstract: Selenium (Se) is an essential element for mammals, and its deficiency in the diet is a global problem. Agronomic biofortification through exogenous Se provides a valuable strategy to enhance human Se intake. Selenium nanoparticles (SeNPs)… read more here.
Keywords: metabolism; soybean; smrt sequencing; mechanism ... See more keywords
Exploring the hepatitis C virus genome using single molecule real-time sequencing
Sign Up to like & getrecommendations! Published in 2019 at "World Journal of Gastroenterology"
DOI: 10.3748/wjg.v25.i32.4661
Abstract: Single molecular real-time (SMRT) sequencing, also called third-generation sequencing, is a novel sequencing technique capable of generating extremely long contiguous sequence reads. While conventional short-read sequencing cannot evaluate the linkage of nucleotide substitutions distant from… read more here.
Keywords: single molecule; real time; smrt sequencing; generation ... See more keywords