Enhancing droplet-based single-nucleus RNA-seq resolution using the semi-supervised machine learning classifier DIEM
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-67513-5
Abstract: Single-nucleus RNA sequencing (snRNA-seq) measures gene expression in individual nuclei instead of cells, allowing for unbiased cell type characterization in solid tissues. We observe that snRNA-seq is commonly subject to contamination by high amounts of… read more here.
Keywords: seq; single nucleus; diem; rna ... See more keywords
Quantification and statistical modeling of droplet-based single-nucleus RNA-sequencing data.
Sign Up to like & getrecommendations! Published in 2023 at "Biostatistics"
DOI: 10.1093/biostatistics/kxad010
Abstract: In complex tissues containing cells that are difficult to dissociate, single-nucleus RNA-sequencing (snRNA-seq) has become the preferred experimental technology over single-cell RNA-sequencing (scRNA-seq) to measure gene expression. To accurately model these data in downstream analyses,… read more here.
Keywords: snrna seq; rna sequencing; seq; single nucleus ... See more keywords
SnRNA-seq and mRNA hybridization indicate key bud events and LcFT1 and LcTFL1-2 mRNA transportability during floral transition in litchi.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of experimental botany"
DOI: 10.1093/jxb/erad103
Abstract: In flowering plants, floral induction signals intersect at the shoot apex to modulate meristem determinacy and growth form. Herein, we reported a snRNA-seq analysis of litchi apical buds at different developmental stages. A total of… read more here.
Keywords: floral transition; snrna seq; lcft1 lctfl1; lctfl1 mrna ... See more keywords
Comparison of single-nucleus and single-cell transcriptomes in hepatocellular carcinoma tissue
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Medicine Reports"
DOI: 10.3892/mmr.2022.12855
Abstract: Single-nucleus RNA sequencing (snRNA-seq) is a method used to analyze gene expression in cells for which isolation is complex, such as those in hepatocellular carcinoma (HCC) tissues. It constitutes an alternative to single-cell RNA sequencing… read more here.
Keywords: cell; snrna seq; scrna seq; seq ... See more keywords
Perspectives on single-nucleus RNA sequencing in different cell types and tissues
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Pathology and Translational Medicine"
DOI: 10.4132/jptm.2022.12.19
Abstract: Single-cell RNA sequencing has become a powerful and essential tool for delineating cellular diversity in normal tissues and alterations in disease states. For certain cell types and conditions, there are difficulties in isolating intact cells… read more here.
Keywords: snrna seq; rna sequencing; single nucleus; cell types ... See more keywords