CryoEM structure of Saccharomyces cerevisiae U1 snRNP offers insight into alternative splicing
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01241-9
Abstract: U1 snRNP plays a critical role in 5ʹ-splice site recognition and is a frequent target of alternative splicing factors. These factors transiently associate with human U1 snRNP and are not amenable for structural studies, while… read more here.
Keywords: saccharomyces cerevisiae; cryoem structure; snrnp; yeast snrnp ... See more keywords
Sequence-specific RNA recognition by an RGG motif connects U1 and U2 snRNP for spliceosome assembly
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2114092119
Abstract: Significance Pre-messenger RNA (pre-mRNA) splicing is a key regulatory step in gene expression. The splicing reaction is mediated by the spliceosome, a dynamic complex comprising five small nuclear ribonucleoproteins (snRNPs), which assembles onto each intron… read more here.
Keywords: spliceosome assembly; rgg; sequence specific; sequence ... See more keywords
Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac636
Abstract: We recently reported that serine-arginine-rich (SR) protein-mediated pre-mRNA structural remodeling generates a pre-mRNA 3D structural scaffold that is stably recognized by the early spliceosomal components. However, the intermediate steps between the free pre-mRNA and the… read more here.
Keywords: pre mrna; snrnp; mrna structural; structural scaffold ... See more keywords
SART3 associates with a post-splicing complex.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cell science"
DOI: 10.1242/jcs.260380
Abstract: SART3 is a multifunctional protein that acts in several steps of the gene expression including assembly and recycling of spliceosomal U4/U6 small nuclear ribonucleoprotein particle (snRNP). In this work, we provide evidence that SART3 associates… read more here.
Keywords: post splicing; associates post; sart3 associates; splicing complex ... See more keywords
Phylogenetic Comparison and Splicing Analysis of the U1 snRNP-specific Protein U1C in Eukaryotes
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2021.696319
Abstract: As a pivotal regulator of 5’ splice site recognition, U1 small nuclear ribonucleoprotein (U1 snRNP)-specific protein C (U1C) regulates pre-mRNA splicing by interacting with other components of the U1 snRNP complex. Previous studies have shown… read more here.
Keywords: snrnp; protein u1c; analysis; specific protein ... See more keywords